Nov 5, 2018

Nitric oxide is important for sensing and survival under hypoxia in Arabidopsis

BioRxiv : the Preprint Server for Biology
Aakanksha WanyKapuganti Jagadis Gupta

Abstract

Nitric oxide (NO) is a free radical molecule that plays an important role in hypoxic stress. We studied the impact of hypoxia-induced NO production on the expression of genes and production of metabolites involved in carbon, nitrogen and antioxidant metabolism using wild type (WT) and non-symbiotic haemoglobin-overexpressing (Hb+) and nitrate reductase double mutant (nia1,2) and application of NO scavenger cPTIO of Arabidopsis. We found that imposing hypoxia leads to the increase of NO and reactive oxygen species (ROS) levels in WT, while the reduced levels of NO and higher levels of ROS were observed in roots of Hb+ and nia1,2 mutant. Expression of the genes encoding group VII ERFs and the enzymes involved in fermentative pathways, activities of these enzymes and metabolite levels were highly induced in WT suggesting that NO plays a role in the induction of fermentation. Several genes and metabolites involved in the TCA cycle were induced in WT in comparison to Hb+ and nia mutant line suggesting that NO can accelerate TCA cycle to regenerate reducing equivalents under hypoxia. Interestingly, we found that the genes and metabolites involved in the ascorbate-glutathione cycle were modulated by NO under hypoxia. The alternative o...Continue Reading

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Mentioned in this Paper

Metabolic Process, Cellular
Biochemical Pathway
Genes
Enzymes, antithrombotic
Arabidopsis
Glutathione dehydrogenase (ascorbate)
Antioxidants
Free Radicals
Molecular_function
Response to Hypoxia

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