Nitric oxide is synthesized in acute leukemia cells after exposure to phenolic antioxidants and initially protects against mitochondrial membrane depolarization

Cancer Letters
Christian Kellner, S J Zunino

Abstract

We investigated the early events involved in loss of mitochondrial membrane potential (DeltaPsi(mt)) leading to apoptosis in cells derived from patients with acute lymphocytic leukemia after exposure to phenolic antioxidants. Using the nitric oxide binding dye diaminofluorescein-FM diacetate, we found that intracellular nitric oxide (NO) levels increased significantly within 4h after exposure to the antioxidants curcumin, carnosol, and quercetin. Inhibition of nitric oxide synthetase (NOS) activity with mercaptoethylguanidine increased the percentage of leukemia cells with depolarized mitochondria membranes after antioxidant treatment. These data suggest that NO production in the leukemia-derived cells may be a protective response to maintain DeltaPsi(mt) after antioxidant exposure and inhibition of NOS increases the disruption of mitochondrial homeostasis induced by the antioxidants.

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Citations

Jul 20, 2007·Apoptosis : an International Journal on Programmed Cell Death·Shashi BhushanJaswant Singh
Jun 1, 2005·Trends in Pharmacological Sciences·Chi Chen, Ah-Ng Tony Kong
Oct 25, 2008·Critical Reviews in Food Science and Nutrition·Gregory Tucker, Kevin Robards
May 9, 2008·Cancer Letters·Preetha AnandBharat B Aggarwal
Feb 14, 2019·Journal of Clinical Medicine·Rosamaria CapuanoCorrado Di Natale
Jun 26, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Tarunpreet Singh ThindSaroj Arora
Feb 14, 2007·Journal of Agricultural and Food Chemistry·Yi-Hsuan ChenGow-Chin Yen

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