Nitric oxide-mediated cytotoxic effects of alveolar macrophages on transformed lung epithelial cells are independent of the beta 2 integrin-mediated intercellular adhesion

Immunology
S Hirano

Abstract

It is known that murine macrophages produce nitric oxide (NO) when stimulated with lipopolysaccharide (LPS) or interferon-gamma (IFN-gamma), and NO mediates the tumoricidal activity of activated macrophages. The present study was designed to investigate whether the intercellular adhesion is necessary for activated rat alveolar macrophages to exert the full cytotoxic effects. Rat alveolar macrophages produced NO dose dependently in response to either LPS or IFN-gamma, and caused DNA fragmentation in rat type II pneumocytes transformed with SV40 (SV40T2). Chemically produced NO also caused the DNA fragmentation and viability loss in SV40T2, and both of them were inhibited by a NO radical scavenger. The cytotoxicity of activated macrophages was reduced by NG-monomethyl-L-arginine, a competitive nitric synthase inhibitor, and neither superoxide dismutase nor catalase modulated the cytotoxicity. Although alveolar macrophages stimulated with either LPS or IFN-gamma caused DNA fragmentation of SV40T2, only LPS increased the intercellular adherence between macrophages and SV40T2. The intercellular adhesion was reduced by both anti-CD18 and anti-CD11a. However, those antibodies did not affect the cytotoxicity of LPS-stimulated macrophag...Continue Reading

References

Sep 4, 1992·Cell·C J Lowenstein, S H Snyder
Dec 1, 1992·Journal of Leukocyte Biology·J T SummersgillJ A Ramirez
Feb 1, 1990·The American Review of Respiratory Disease·Y Sibille, H Y Reynolds
Oct 1, 1989·Fundamental and Applied Toxicology : Official Journal of the Society of Toxicology·C P YuP E Morrow
Apr 1, 1988·The Journal of Clinical Investigation·D L GrangerD T Durack
Jan 1, 1985·International Archives of Allergy and Applied Immunology·R van Furth
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·D J Stuehr, M A Marletta
Feb 1, 1994·American Journal of Respiratory and Critical Care Medicine·B GastonJ S Stamler
Nov 19, 1993·Cell·P C Doherty
Apr 17, 1995·Biochemical and Biophysical Research Communications·C Szabó, A L Salzman
Sep 23, 1994·Cell·H H Schmidt, U Walter
Sep 24, 1996·Biochemical and Biophysical Research Communications·F F Behar-CohenO Goureau

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Citations

Sep 6, 2000·American Journal of Respiratory Cell and Molecular Biology·S HiranoS Kanno
Apr 18, 2003·Clinical and Experimental Immunology·M-J TherriaultE Y Bissonnette
Sep 3, 2004·Critical Care Medicine·Alain F BroccardMarie-Denise Schaller
Aug 16, 2003·Laboratory Investigation; a Journal of Technical Methods and Pathology·Ayako YoshidaVictor M Elner
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y S EdwardsA W Murray
Apr 6, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·S PepperlF Krombach
Jun 9, 2001·Antioxidants & Redox Signaling·J D LaskinD L Laskin
Oct 12, 2020·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Shugo YamashitaAkira Yamamoto
May 23, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Y S Edwards

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