Nitric oxide-mediated death of cultured neonatal retinal ganglion cells: neuroprotective properties of glutamate and chondroitin sulfate proteoglycan

Brain Research
K A NicholM R Bennett

Abstract

The release of nitric oxide and stimulation of glutamate receptors by excitatory amino acids has been linked to neuronal degeneration and toxicity. In the rat retina approximately 60% of retinal ganglion cells (RGCs) die during the first postnatal week. In this study we examined the effects of nitric oxide synthase blockers and glutamate on the survival of neonatal RGCs in vitro over a 16 h assay period. Less than 10% of P1 RGCs survived in serum free defined media alone (control), however survival was increased, in a dose-dependent manner, when L-glutamate (10 microM-10 mM) was added to the media; a maximum of 70% of RGCs could be maintained with the addition of 5 mM glutamate. This effect was blocked by the NMDA and non-NMDA receptor blockers APV and DNQX and was age dependent; the survival of RGCs from P5 but not P7 rats was enhanced by the addition of glutamate even in high calcium concentrations (10 mM). When the nitric oxide synthase blockers L-NAME (5 mM) or haemoglobin (25 microM) were added to the culture media, up to 61% of P1 RGCs survived. The addition of the 480 kDa chondroitin sulfate proteoglycan (SCCP) previously shown to enhance RGC survival in vivo and in vitro, potentiated the action of glutamate and L-NAME a...Continue Reading

References

Jun 19, 1992·Brain Research. Developmental Brain Research·N A Rashid, M A Cambray-Deakin
Jan 1, 1992·Neuron·D S Bredt, S H Snyder
Jan 1, 1992·Neuroscience·S R Vincent, H Kimura
Jun 5, 1992·European Journal of Pharmacology·C ChiamuleraA Reggiani
Mar 20, 1992·Brain Research. Developmental Brain Research·M A Cambray-Deakin, R D Burgoyne
May 3, 1991·Brain Research·K FunkeT FitzGibbon
Feb 1, 1991·Trends in Neurosciences·J Garthwaite
Oct 31, 1991·Biochemical and Biophysical Research Communications·C M VenturiniS Moncada
Nov 27, 1991·Biochemical and Biophysical Research Communications·C Demerlé-PallardyP Braquet
Jan 11, 1992·Experimental Neurology·E M JohnsonJ Franklin
Jan 1, 1990·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·A Frandsen, A Schousboe
Jan 1, 1990·Journal of Neurophysiology·S C Massey, R F Miller
Apr 1, 1990·Brain Research. Developmental Brain Research·M A Cambray-DeakinR D Burgoyne
Sep 1, 1990·Journal of Neurochemistry·M SchulzM R Bennett
Sep 1, 1990·Trends in Pharmacological Sciences·B Meldrum, J Garthwaite
Oct 17, 1989·European Journal of Pharmacology·J GarthwaiteS Moncada
Jul 31, 1989·Neuroscience Letters·J Mitrofanis
Jan 1, 1986·Advances in Experimental Medicine and Biology·S M Rothman
Sep 22, 1987·The Journal of Comparative Neurology·G M Horsburgh, A J Sefton
Jun 24, 1985·Neuroscience Letters·C A McCafferyM R Bennett
Aug 22, 1985·The Journal of Comparative Neurology·J H Sandell
Mar 1, 1982·Brain Research·R A PottsM R Bennett

❮ Previous
Next ❯

Citations

Feb 24, 2001·International Ophthalmology Clinics·R H Farkas, C L Grosskreutz
Jul 26, 2002·Journal of Glaucoma·Kenji KashiwagiShigeo Tsukahara
Jan 28, 2004·Journal of Pharmacological Sciences·Hiroshi KatsukiAkinori Akaike
Mar 7, 2017·Scientific Reports·Jacqueline ReinhardStephanie C Joachim
Jun 30, 2006·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J RumbaughA Nath
Jul 23, 1999·Survey of Ophthalmology·N N OsborneM S Nash
Sep 5, 2002·Progress in Retinal and Eye Research·Masaru Inatani, Hidenobu Tanihara
May 19, 2004·Biochemical and Biophysical Research Communications·Peter KabosRichard C Krueger
Apr 23, 2005·Progress in Retinal and Eye Research·Rafael LindenMariana S Silveira

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.