Nitric Oxide-Mediated Maize Root Apex Responses to Nitrate are Regulated by Auxin and Strigolactones

Frontiers in Plant Science
Alessandro ManoliSilvia Quaggiotti

Abstract

Nitrate (NO3 (-)) is a key element for crop production but its levels in agricultural soils are limited. Plants have developed mechanisms to cope with these NO3 (-) fluctuations based on sensing nitrate at the root apex. Particularly, the transition zone (TZ) of root apex has been suggested as a signaling-response zone. This study dissects cellular and molecular mechanisms underlying NO3 (-) resupply effects on primary root (PR) growth in maize, confirming nitric oxide (NO) as a putative modulator. Nitrate restoration induced PR elongation within the first 2 h, corresponding to a stimulation of cell elongation at the basal border of the TZ. Xyloglucans (XGs) immunolocalization together with Brefeldin A applications demonstrated that nitrate resupply induces XG accumulation. This effect was blocked by cPTIO (NO scavenger). Transcriptional analysis of ZmXET1 confirmed the stimulatory effect of nitrate on XGs accumulation in cells of the TZ. Immunolocalization analyses revealed a positive effect of nitrate resupply on auxin and PIN1 accumulation, but a transcriptional regulation of auxin biosynthesis/transport/signaling genes was excluded. Short-term nitrate treatment repressed the transcription of genes involved in strigolactones...Continue Reading

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Citations

Feb 27, 2018·Physiologia Plantarum·Zsuzsanna Kolbert
May 31, 2019·Plant & Cell Physiology·Laura RavazzoloSilvia Quaggiotti
Jun 8, 2018·Frontiers in Plant Science·Huwei SunQuanzhi Zhao
Aug 8, 2018·Journal of Experimental Botany·Mari-Cruz CastilloJosé León
Jul 29, 2020·Frontiers in Plant Science·Dóra OláhZsuzsanna Kolbert
Dec 6, 2018·Frontiers in Plant Science·Guodong ChenShutian Tao
Dec 2, 2019·Plant Physiology and Biochemistry : PPB·Lili ZangFrançoise Montrichard
Jun 15, 2021·Frontiers in Plant Science·Inmaculada Sánchez-VicenteOscar Lorenzo

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Methods Mentioned

BETA
RSA
RNA-Seq
PCR
GTPase

Software Mentioned

Primer3
PRATO
Adobe Photoshop
Image J Image Analysis

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