Nitric oxide mediates inhibitory effect of leptin on insulin-like growth factor I augmentation of 17beta-estradiol production in human granulosa cells

Biology of Reproduction
He-Feng HuangJian-Zhong Sheng

Abstract

In the present study the authors investigated if the inhibitory effect of leptin in the ovary was mediated via nitric oxide (NO) using human granulosa cells (GCs). Human GCs were obtained from preovulatory follicles of women who underwent IVF. Reverse transcription-polymerase chain reaction (RT-PCR) demonstrated that human GCs expressed mRNA of leptin and mRNA of isoforms of leptin receptor, including one long form and two types of short forms. Exposure of human GCs to leptin at concentrations of 3-30 ng/ml for 60 min dose-dependently increased the fluorescence of 4,5-diaminofluorescein (DAF-2), an NO-sensitive dye. The effect of leptin on DAF-2 fluorescence was inhibited by pretreatment of human GCs with 100 microM nitro-L-arginine methyl ester (L-NAME), an inhibitor of NO synthase (NOS), indicating that the increase in DAF-2 fluorescence properly reflected the intracellular NO production. FSH (1 ng/ ml) and IGF-I (30 ng/ml) stimulated 17beta-estradiol (E2) production in human GCs, respectively. FSH plus IGF-I induced a further increase in E2 production. Leptin did not significantly alter basal or FSH-dependent E2 production, but it inhibited the effect of IGF-I on E2 production and the synergistic effect of IGF-I on FSH-stimu...Continue Reading

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Citations

Mar 12, 2008·Histochemistry and Cell Biology·Thomas SchmidtBernd Fischer
Jun 1, 2006·Current Opinion in Obstetrics & Gynecology·Ana CerveroCarlos Simón
Oct 31, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·A G RicciA G Faletti
May 6, 2011·Reproduction : the Official Journal of the Society for the Study of Fertility·Miguel A VelazquezHeiner Niemann
Sep 1, 2015·Canadian Journal of Physiology and Pharmacology·Anna T Grazul-BilskaDale A Redmer
Dec 25, 2012·Research in Veterinary Science·C TrisoliniR L Sciorsci
Jul 11, 2009·Fertility and Sterility·Henry BohlerStephen J Winters
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Jun 1, 2006·Current Opinion in Obstetrics & Gynecology
Oct 18, 2007·Current Opinion in Endocrinology, Diabetes, and Obesity

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BETA
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Axonscope

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