Nitric oxide mediates inhibitory effect of interleukin-1beta on estrogen production in human granulosa-luteal cells

The Journal of Obstetrics and Gynaecology Research
H Tobai, I Nishiya

Abstract

To investigate the effect of IL-1beta on NO production and steroidogenesis in human granulosa-luteal cells obtained from women undergoing in vitro fertilization procedures. To investigate the effect of IL-1beta, granulosa-luteal cells were cultured with various doses of IL-1beta (0, 0.05, 0.5, 5, 50, 100 ng/ml), IL-1beta (5 ng/ml) with NG-nitro-L-arginine-methyl ester (L-NAME), selective inhibitors of NOS, sodium nitroprusside (SNP), NO donors and Genistain, a tyrosine kinase inhibitor. IL-1beta induced a dose-dependent stimulation of NO production and inhibited the production of estradiol in a significant way in a dose-dependent manner. L-NAME significantly decreased NO production and increased the production of estradiol and progesterone. SNP significantly increased NO production and caused decreases in the production of both estradiol and progesterone. Genistain decreased NO production and significantly increased the production of estradiol and progesterone. Inducible NOS (iNOS) messenger RNA was present in granulosa-luteal cells before treatment with IL-1beta. IL-1beta stimulated NO production, and NO inhibited the production of estradiol.

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Citations

Sep 1, 2015·Canadian Journal of Physiology and Pharmacology·Anna T Grazul-BilskaDale A Redmer
Mar 21, 2015·Reproduction : the Official Journal of the Society for the Study of Fertility·Giuseppina Basini, Francesca Grasselli
Nov 16, 2007·The Journal of Obstetrics and Gynaecology Research·Hiroshi AnbeRobert E Garfield
Mar 13, 2014·Advances in Nutrition·Rosemary L Walzem, Shuen-ei Chen
Apr 14, 2019·Reproductive Biology and Endocrinology : RB&E·Yiran LiYingpu Sun
Dec 14, 2018·Experimental and Therapeutic Medicine·Hui WangLi Sheng
Mar 11, 2003·Reproductive Biomedicine Online·Philip S LaPoltJean You-hsin Chen

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