PMID: 8589348Jan 1, 1995Paper

Nitric oxide mediates mitochondrial dysfunction in hepatoma cells induced by non-activated Kupffer cells: evidence implicating ICAM-1-dependent process

Journal of Gastroenterology and Hepatology
I KuroseH Ishii

Abstract

The metabolic changes in a rat hepatoma cell line, AH70 cells, after co-culture with rat Kupffer cells (KC) were visualized and analysed using a fluorescence microscope equipped with a silicon intensified target camera and a laser scanning confocal microscopic system. Kupffer cells were isolated from male Wistar rats, and cultured without any stimuli. The non-activated KC reduced the mitochondrial energization in the cocultured AH70 cells within 2 h, which was indicated by decreased rhodamine 123 (Rh123) fluorescence. Either NG-monomethyl-L-arginine or dexamethasone significantly attenuated the KC-induced mitochondrial dysfunction in AH70 cells, suggesting the involvement of nitric oxide (NO) derived from inducible-type nitric oxide synthase (iNOS). Administration of monoclonal antibody (mAb) directed against rat ICAM-1 also prevented the decrease in Rh123 fluorescence. Electron microscopy revealed that the membrane-to-membrane attachment between KC and AH70 cells occurred within 2 h. A laser scanning confocal microscopic observation using mAb against ICAM-1 presented that the ICAM-1 expression on AH70 cells and KC increased after the co-culture. It is therefore concluded that the KC-mediated mitochondrial dysfunction of hepato...Continue Reading

References

Jul 1, 1992·Acta Physiologica Scandinavica·S Moncada
Sep 1, 1989·Biological Chemistry Hoppe-Seyler·G LatochaK Decker

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Citations

Apr 24, 2001·Free Radical Biology & Medicine·N WatanabeH Ishii
May 9, 2015·Seminars in Cancer Biology·Abbas K SamadiXujuan Yang
Jun 7, 2003·Inflammatory Bowel Diseases·Raymond K Cross, Keith T Wilson
Jan 4, 1998·Journal of Gastroenterology and Hepatology·Y F Liaw

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