PMID: 8584441Jan 1, 1996Paper

Nitric oxide mediates the anti-adrenergic effect of adenosine on calcium current in isolated rabbit atrioventricular nodal cells

Pflügers Archiv : European journal of physiology
A E MartynyukA C Rankin

Abstract

The aim of this study was to determine if adenosine exerts an anti-adrenergic effect on rabbit isolated atrioventricular (AV) nodal cells and, if so, the dependence of this effect on nitric oxide (NO) production. Inward Ca current, ICa, was measured in AV nodal cells, enzymatically isolated from rabbit hearts. Isoprenaline (0.1 microM) increased ICa from 676 +/-59 to 1102 +/-86 pA (n =25). This isoprenaline-induced increase in ICa(178 +/-15% of control) was abolished in the presence of 10 microM adenosine (ICa100 +/-2% of control, n =9, P <0.05). This effect of adenosine was completely blocked by the A1 receptor antagonist CPDPX (8-cyclopentyl l, 3-dipropylxanthine, 0.1 microM). In cells pre-treated with the NO synthase inhibitor, L-nitro-arginine methyl ester (L-NAME, 1 mM) the isoprenaline-induced increase in ICa(208 +/-39% of control, n=7) was not reduced by the addition of 10 microM adenosine (195 +/-32% of control). Co-incubation of cells in L-NAME with L-arginine (1 mM, the endogenous substrate of NO synthase) restored the adenosine-induced attenuation of ICa. In these cells, isoprenaline increased ICa (157 +/-7% of control, n =6), and, following addition of adenosine (10 microM) ICa was reduced to 107 +/-8% (P <0.05). Th...Continue Reading

References

Dec 1, 1990·Journal of Molecular and Cellular Cardiology·A C RankinK A Kane
Jun 1, 1987·The Journal of Physiology·R Fischmeister, H C Hartzell
Jul 1, 1989·Progress in Cardiovascular Diseases·L BelardinelliR M Berne
Mar 1, 1994·Pflügers Archiv : European journal of physiology·R C LeviM P Gallo
Jan 1, 1995·The American Journal of Physiology·G M Wahler, S J Dollinger
Jan 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·J L BalligandT Michel
Apr 15, 1994·The Journal of Physiology·X HanW R Giles
Jan 1, 1993·Physiological Reviews·H IrisawaW Giles
Jan 1, 1993·Cardiovascular Research·A Pelleg, L Belardinelli

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Citations

Jun 19, 1997·The American Journal of Cardiology·J C Shryock, L Belardinelli
Oct 24, 1998·Progress in Biophysics and Molecular Biology·E Carmeliet, K Mubagwa
Jun 6, 2000·Brain Research. Developmental Brain Research·C K BarcellosA C Webb
Oct 19, 2001·British Journal of Pharmacology·P NieriM C Breschi
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·X HanR A Kelly
Dec 25, 2015·Heart Failure Reviews·Stephen J GreeneMihai Gheorghiade
May 23, 2014·The Journal of Sexual Medicine·Linda VignozziMario Maggi
Nov 1, 2016·Expert Review of Cardiovascular Therapy·Hani N Sabbah
Oct 1, 1998·Journal of Cardiac Failure·M A Arstall, R A Kelly
Dec 19, 2001·Microscopy Research and Technique·W BlochB K Fleischmann
Sep 1, 1996·Circulation Research·R A KellyT W Smith
Oct 17, 2003·American Journal of Physiology. Heart and Circulatory Physiology·John P HeadrickKevin J Ashton
Mar 22, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Qinghang Liu, Polly A Hofmann

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