Nitric oxide microsensor for high spatial resolution measurements in biofilms and sediments

Analytical Chemistry
Frank SchreiberDirk de Beer

Abstract

Nitric oxide (NO) is a ubiquitous biomolecule that is known as a signaling compound in eukaryotes and prokaryotes. In addition, NO is involved in all conversions of the biogeochemical nitrogen cycle: denitrification, nitrification, and the anaerobic oxidation of ammonium (Anammox). Until now, NO has not been measured with high spatial resolution within microbial communities, such as biofilms, sediments, aggregates, or microbial mats, because the available sensors are not robust enough and their spatial resolution is insufficient. Here we describe the fabrication and application of a novel Clark-type NO microsensor with an internal reference electrode and a guard anode. The NO microsensor has a spatial resolution of 60-80 microm, a sensitivity of 2 pA microM-1, and a detection limit of approximately 30 nM. Hydrogen sulfide (H2S) was found to be a major interfering compound for the electrochemical detection of NO. The application of the novel NO microsensor to nitrifying biofilms and marine sediments revealed dynamic NO concentration profiles with peaks in the oxic parts of the samples. The local concentrations suggested that NO may be an important bioactive compound in natural environments. The consumption and production of NO o...Continue Reading

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Citations

May 19, 2010·Analytical Chemistry·Benjamin J PrivettMark H Schoenfisch
Feb 3, 2012·Environmental Science & Technology·Sarina JenniKai M Udert
Jun 12, 2009·The ISME Journal·Frank SchreiberDirk de Beer
Mar 24, 2010·BMC Biology·Frank SchreiberPaul Stoodley
Aug 8, 2017·Biotechnology and Bioengineering·Fabrizio SabbaRobert Nerenberg
Aug 13, 2013·Journal of Bacteriology·Andrew M SternJun Zhu
Mar 26, 2010·Nature·Katharina F EttwigMarc Strous
Sep 12, 2018·Applied Microbiology and Biotechnology·Fabrizio SabbaRobert Nerenberg
Jul 22, 2020·Analytical and Bioanalytical Chemistry·Giada Caniglia, Christine Kranz
Mar 6, 2019·The Review of Scientific Instruments·Sheng Zhou, Davide Iannuzzi
Apr 22, 2018·Environmental Science & Technology·Rathnayake M L D RathnayakeSatoshi Okabe
Oct 16, 2014·Environmental Science & Technology·Ricardo MarquesMaite Pijuan

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