Nitric oxide modulates sympathetic neurotransmission at the prejunctional level

Microscopy Research and Technique
K AddicksM Feelisch

Abstract

In spite of accumulating evidence for a modulation of sympathetic neurotransmission by endogenously produced nitric oxide (NO), it remains unclear in which parts of the vascular system and at what level this interaction takes place. The aim of the present study was to investigate the distribution of endothelial and neuronal NO synthase (NOS) along the vascular tree of the heart at the light and electron microscopic level using NADPH-diaphorase (NADPH-d) staining as a marker for NOS. In addition, the functional effects of exogenous NO on coronary vascular resistance and cardiac adrenergic nerves was studied using the isolated perfused rat heart as a model. The intraaxonal catecholamine content of adrenergic nerve fibers was visualised and morphometrically assessed by applying glyoxylic acid-induced histofluorescence. The expression of endothelial NOS in the heart was found to depend on the diameter of the blood vessel. Arteries > 100 microns always showed intense staining, whereas staining in smaller arteries and veins was considerably weaker. Smooth-muscle free vessels were essentially devoid of NADPH-d activity. In atrial and ventricular myocardium, neuronal NOS localised in autonomic nerve fibers along the entire vascular tre...Continue Reading

References

Jun 1, 1992·Circulation Research·M UeedaR A Olsson
Jul 17, 1992·Science·A L BurnettS H Snyder
May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C RamaciottiS Winegrad
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·B T HopeS R Vincent
Jan 1, 1991·Journal of Molecular and Cellular Cardiology·A M ShahA H Henderson
Apr 1, 1991·Trends in Pharmacological Sciences·S H Snyder, D S Bredt
Jun 1, 1990·Circulation Research·M Kelm, J Schrader
Nov 1, 1990·Circulation·M Akhtar
Aug 1, 1986·British Journal of Pharmacology·J A AngusK Satoh
Mar 14, 1989·European Journal of Pharmacology·S GreenbergT P Tanaka
Sep 18, 1989·The American Journal of Medicine·P M Vanhoutte, V M Miller
Oct 1, 1980·Journal of Neuroscience Methods·J C De la Torre
Jan 1, 1964·The American Journal of Medicine·E BRAUNWALDC A CHIDSEY

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Citations

May 5, 2000·Journal of Nuclear Cardiology : Official Publication of the American Society of Nuclear Cardiology·H FujitaM Yokoyama
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·T LauerM Kelm
Aug 4, 2001·Nitric Oxide : Biology and Chemistry·W BlochK Addicks
Jun 4, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·M GerováM Cebová
Mar 23, 2005·Neuroscience and Behavioral Physiology·M A GilinskiiV V Bulygina
Dec 19, 2001·Microscopy Research and Technique·W BlochB K Fleischmann
Feb 26, 2015·Journal of Hypertension·Alice Victoria Klein, Hosen Kiat
Jun 15, 1999·Journal of Cardiovascular Pharmacology·S ArnholdK Addicks
Oct 31, 1998·Journal of Hypertension·A Sánchez-MendozaB Escalante
Aug 13, 2004·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Claire E SearsBarbara Casadei
Jul 28, 2001·CNS Drug Reviews·A Ottani, D Giuliani
Oct 25, 2016·Physiological Research·A Puzserova, I Bernatova

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