Nitric oxide modulation of early angiogenesis

Microsurgery
J Scott Isenberg

Abstract

Angiogenesis is the process of new vessel formation from an existing vasculature network. In all but a few circumstances it is tightly controlled and suppressed. Precise understanding of the factors involved in modulation of angiogenesis has significant potential clinical value. One agent believed to play a role in angiogenesis is nitric oxide. However, there remain substantial uncertainties concerning the specifics of this role. The present study was undertaken to better define the role nitric oxide plays in angiogenesis associated with acute wound healing. Muscle biopsies from the pectoralis major of C57B6 mice were embedded in 500 microl of type I collagen matrix, and incubated in the presence of growth medium for 14 days. Treatment wells received L-Arginine (2 mM), L-NAME (300 microM), or SNAP (10-20 microM). Angiogenic response was quantified as the measure of cell migration through the matrix and as the total cells recovered from the matrix. Whole lung specimens and aortic segments served as sources of endothelial and vascular smooth muscle cells respectively for proliferation studies under similar treatment conditions. Nitric oxide was found to exert either a stimulatory or inhibitory effect on angiogenesis and cell prol...Continue Reading

References

Feb 23, 1999·Clinical and Experimental Pharmacology & Physiology·D V Faller
Mar 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·L J IgnarroP del Soldato
Sep 7, 2001·Current Opinion in Cell Biology·B P Eliceiri, D A Cheresh
Mar 23, 2002·Circulation Research·L González-SantiagoD Rodríguez-Puyol
Jan 4, 2003·Hypertension·Katsuya AmanoToshiji Iwasaka
Feb 13, 2003·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Rainer MittermayrHeinz Redl
Mar 6, 2003·Brain Research·Xueren ZhaoCostantino Iadecola
Oct 10, 2003·British Journal of Pharmacology·Thomas N Sato
Nov 1, 2003·The International Journal of Biochemistry & Cell Biology·Shunichi ShimizuYuji Kiuchi

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Citations

Jul 21, 2005·Microsurgery·J S IsenbergHenry M Spinelli
Oct 27, 2006·Annals of Biomedical Engineering·Ayman HamedMichael Cho
Mar 9, 2005·Biochemical and Biophysical Research Communications·Chunying LiRichard C Venema
Dec 25, 2007·Annals of Surgery·Jeff S IsenbergDavid D Roberts
Dec 23, 2006·Acta Pharmacologica Sinica·Zhen-Zhong Zheng, Zheng-Xiang Liu
Apr 12, 2006·Photodermatology, Photoimmunology & Photomedicine·Shi-Yau YuChew-Wun Wu
Mar 17, 2006·Free Radical Biology & Medicine·Jeff S IsenbergMichael Graham Espey
Jan 12, 2007·Journal of the American Society of Nephrology : JASN·Bernd HohensteinChristian P M Hugo

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