Nitric oxide modulation of pulmonary vascular resistance is red blood cell dependent in isolated rat lungs

Anesthesia and Analgesia
D R UnclesG F Rich

Abstract

Nitric oxide (NO) or endothelium-derived relaxing factor may play an important role in modulating pulmonary vascular resistance (PVR), although previous studies have produced conflicting results. Endogenous NO inhibition causes an increase in PVR in intact animals but not in saline-perfused isolated lungs. We hypothesized that blood is essential for NO to serve as a modulator of PVR. Therefore, the effects of endogenous NO inhibition (N omega-nitro-L-arginine methyl ester [L-NAME]) were determined in isolated rat lungs as related to the presence of different blood components under normoxic conditions and after 1 wk of hypoxia (fraction of inspired oxygen [FIO2] = 10%). Exogenously administered inhaled NO was evaluated in isolated lungs from normoxic and hypoxic rats. In normoxic rats, L-NAME (10-100 microM) caused a dose-dependent increase in PVR in whole (hematocrit [Hct] 40%) and diluted (Hct 12%) blood-perfused lungs. L-NAME (10-800 microM) had no effect in isolated lungs perfused with a modified salt solution of equal viscosity to blood either alone, or containing plasma (50%) or free oxyhemoglobin (10 microM). In whole blood perfused lungs, L-NAME (100 microM) increased PVR more in hypoxic versus normoxic isolated lungs (1...Continue Reading

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Citations

Mar 3, 2004·Free Radical Biology & Medicine·Steven Deem
May 14, 1999·Drug Discovery Today·D DiSepioS Nagpal
Jan 3, 2013·The Journal of Clinical Investigation·Saptarsi M Haldar, Jonathan S Stamler
May 12, 2004·The Journal of Heart and Lung Transplantation : the Official Publication of the International Society for Heart Transplantation·Takayuki NakamuraHans-Joachim Schäfers
Dec 14, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Griffith LiangRandy S Sprague
Mar 22, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Fabian SpöhrJörg Weimann
Sep 19, 2020·Experimental Lung Research·Maurizio TurzoCornelius J Busch

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