Nitric oxide (NO) inhibits prostaglandin E2 9-ketoreductase (9-KPR) activity in human fetal membranes

Prostaglandins & Other Lipid Mediators
M G FarinaA M Franchi

Abstract

Nitric oxide (NO) synthesized by fetal membranes may act either directly inhibiting myometrium contractility or indirectly interacting with tocolytic agents as prostaglandins (PGs). Here we examined if NO could modulate prostaglandin E(2) 9-ketoreductase (9-KPR) activity in human fetal membranes (HFM). 9-KPR is the enzyme that converts PGE(2) into PGF(2alpha), the main PGs known to induce uterine contractility at term. Chorioamnion explants obtained from elective caesareans were incubated with aminoguanidine (AG), an iNOS inhibitor, or NOC-18, a NO donor. NOC-18 (2mM) increased PGE(2) production and diminished PGF(2alpha) synthesis in HFM. AG presented the opposite effect. When we evaluated the activity of 9-KPR by the conversion of [(3)H]-PGE(2) into [(3)H]-PGF(2alpha) and 13,14-dihidro-15-keto prostaglandin F(2alpha) (the PGF(2alpha) metabolite), we found that NOC-18 inhibited 9-KPR activity. Interestingly, AG did not elicit any effect on 9-KPR but l-NAME, a non-selective NOS inhibitor, significantly increased its activity. Our data suggests that exogenous NO inhibits 9-KPR activity in HFM, thus modulating the synthesis of important labor mediators as PGF(2alpha).

References

Feb 1, 1978·Prostaglandins·M D MitchellA C Turnbull
Apr 1, 1985·British Journal of Obstetrics and Gynaecology·S P BrenneckeA C Turnbull
Jan 1, 1983·Acta Obstetricia Et Gynecologica Scandinavica. Supplement·N WiqvistL Wilhelmsson
Mar 1, 1995·British Journal of Pharmacology·D SalveminiP Needleman
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·A M FranchiM Gimeno
Nov 5, 1997·Acta Obstetricia Et Gynecologica Scandinavica·E MarinoniE V Cosmi
Nov 14, 1997·American Journal of Reproductive Immunology : AJRI·A L EisL Myatt
Apr 9, 1999·Journal of Molecular Endocrinology·D SlaterP Bennett
Dec 4, 2003·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M L RibeiroA Franchi

❮ Previous
Next ❯

Citations

Oct 10, 2008·Reproduction, Fertility, and Development·Valeria A SanderAlicia B Motta
Apr 29, 2015·Experimental Animals·Lu YangChengzhi Chai
Sep 22, 2009·Prostaglandins & Other Lipid Mediators·Y S WeemsC W Weems
Sep 3, 2009·Prostaglandins & Other Lipid Mediators·Y S WeemsC W Weems
Jun 22, 2007·Seminars in Cell & Developmental Biology·A López Bernal
Jul 24, 2007·Prostaglandins & Other Lipid Mediators·Yoshie S WeemsCharles W Weems

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.