Nitric oxide prevents human platelet adhesion to fiber membranes in whole blood

ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs
R KonishiB G Hattler

Abstract

During cardiopulmonary bypass or long-term extracorporeal life support, foreign surface induced platelet deposition in the oxygenator causes deterioration of gas exchange. In this study, the authors evaluated the effectiveness of nitric oxide (NO) in reducing the adhesion of platelets in whole blood to the surface of hollow fiber membranes. For this purpose, a test chamber was designed consisting of a gas exchanger with ten mitsubishi multi-layered composite hollow fibers (MHF: 257 mm OD; 203 mm ID; 70 mm length) and a polypropylene tube (16 mm OD; 100 mm length). Pure N2 (control) or nitric oxide (NO) (100 ppm, 200 ppm in N2) were delivered into the test chamber previously filled with 13 ml human whole blood. Platelet counts and platelet factor 4 (PF4), as a measure of platelet activation, were measured before and after either 1 or 2 hr of testing, and fibers were observed under scanning electron microscopic study (SEM) after each experiment. In the control and 100 ppm NO groups, platelet counts decreased and the level of PF4 increased during the 1 hr period. In the 200 ppm NO group, almost no platelet deposition could be observed on the surface of fibers under SEM. In conclusion, NO flow through hollow fiber membranes can mar...Continue Reading

Associated Clinical Trials

Citations

Oct 20, 2005·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Satoshi Saito, Tomohiro Nishinaka
Feb 4, 1999·The Annals of Thoracic Surgery·A Weerasinghe, K M Taylor
Jun 30, 2007·Journal of Vascular Surgery·Shen-Kou TsaiShiang-Suo Huang
Apr 10, 2010·Korean Journal of Ophthalmology : KJO·Woo Taek Kim, Eok Soo Suh
Dec 8, 2000·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·H T TevaearaiL K von Segesser
Aug 24, 1999·Perfusion·B G Hattler, W J Federspiel

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