Nitric oxide produced by a novel nitric oxide synthase isoform is necessary for gonadotropin-releasing hormone-induced growth hormone secretion via a cGMP-dependent mechanism

Journal of Neuroendocrinology
A D UretskyJ P Chang

Abstract

The involvement of nitric oxide (NO) in the regulation of goldfish growth hormone (GH) secretion was further characterized using primary cultures of dispersed goldfish pituitary cells. Western blots revealed the presence of an inducible nitric oxide synthase (iNOS)-like protein of approximately 120 kDa in cytosol/plasma membrane extracts. By contrast, brain NOS-immunoreactive proteins of approximately 120-140 kDa were occasionally detected in a cytoskeleton/organelle fraction but were absent from cytosol/plasma membrane extracts. The NO donor sodium nitroprusside (SNP) acutely increased GH secretion but this response was not observed in the presence of either a NO scavenger (PTIO) or a soluble guanylate cyclase inhibitor (ODQ). SNP also significantly increased the levels of cyclic (c)GMP in somatotrope-enriched cell populations. Treatments with 1400W (iNOS inhibitor), PTIO and rutin hydrate (NO scavengers) and ODQ abolished the acute GH-release response to two endogenous gonadotropin-releasing hormones (GnRH). 1400W, rutin hydrate, PTIO and ODQ alone did not significantly alter basal GH secretion. Together, these results establish that an iNOS-like peptide is constitutively present in the pituitary of the goldfish. Furthermore,...Continue Reading

References

Apr 1, 1991·Trends in Pharmacological Sciences·S H Snyder, D S Bredt
Dec 1, 1988·The Journal of Clinical Endocrinology and Metabolism·J Alba-RothK von Werder
May 1, 1994·Neuroimmunomodulation·V RettoriS M McCann
Sep 25, 1995·Biochemical and Biophysical Research Communications·D J HarttC L Chik
Jan 1, 1996·Proceedings of the Society for Experimental Biology and Medicine·L J McDonald, F Murad
Feb 20, 1996·Proceedings of the National Academy of Sciences of the United States of America·M A MoroS Moncada
Jan 1, 1997·Advances in Pharmacology·L G Korkina, I B Afanas'ev
Jan 20, 1998·Trends in Biochemical Sciences·B Mayer, B Hemmens
Aug 4, 1998·Journal of Neuroendocrinology·A Gobbetti, M Zerani
Oct 24, 1998·Domestic Animal Endocrinology·S M McCannW H Yu
Nov 24, 1999·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·F Murad
Jun 14, 2000·General and Comparative Endocrinology·A D Uretsky, J P Chang
Apr 28, 2001·Molecular and Cellular Endocrinology·W K Yunker, J P Chang

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Citations

Nov 1, 2011·General and Comparative Endocrinology·Vance L TrudeauAjoy Basak
Jan 18, 2011·European Journal of Pharmacology·Yao ZhangRaymond M Quock
Apr 4, 2008·Molecular and Cellular Endocrinology·Kyriaki Thermos
May 14, 2005·Annals of the New York Academy of Sciences·F Rodríguez-PachecoM M Malagón
Jul 12, 2011·Nitric Oxide : Biology and Chemistry·Yoshiaki MiyamotoYasuo Watanabe
Sep 1, 2012·General and Comparative Endocrinology·Caleb L Grey, John P Chang
Dec 25, 2012·General and Comparative Endocrinology·Caleb L Grey, John P Chang
Feb 9, 2007·General and Comparative Endocrinology·Luis Fabián CanosaRichard E Peter
Sep 21, 2007·Nature Reviews. Neuroscience·Vittorio CalabreseAnna Maria Giuffrida Stella
May 24, 2008·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Kar-Lai PoonVladimir Korzh
Aug 2, 2017·Metabolic Brain Disease·Anzelle DelportJacobus P Petzer
Feb 9, 2012·Experimental Biology and Medicine·Lloyd L Anderson, Colin G Scanes
Jan 27, 2019·International Journal of Molecular Sciences·Carla CioniMattia Toni
Mar 27, 2004·Experimental Biology and Medicine·Lloyd L AndersonColin G Scanes

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