Nitric oxide produced in the lungs of mice immunized with the radiation-attenuated schistosome vaccine is not the major agent causing challenge parasite elimination

Immunology
P S CoulsonR A Wilson

Abstract

Mice vaccinated with radiation-attenuated cercariae of Schistosoma mansoni exhibit high levels of protection against a challenge with normal larvae. The immune effector mechanism, which operates against schistosomula in the lungs, requires CD4+ T cells capable of producing interferon-gamma (IFN-gamma). This cytokine can stimulate production of nitric oxide (NO), via its ability to up-regulate inducible nitric oxide synthase (iNOS). We have therefore evaluated the potential role of NO in the effector mechanism operating in vaccinated mice. Evidence for the production of NO in the lungs of such animals was obtained from assays on antigen-stimulated airway cell cultures. Enhanced levels of NO, compared with those in cultures from control mice, were detected both after vaccination and after challenge; elevated levels of iNOS mRNA were also present in whole lung after challenge. However, administration of an iNOS inhibitor to vaccinated mice after percutaneous challenge did not significantly increase the worm burden. Furthermore, when mice with a disrupted iNOS gene were vaccinated they showed a highly significant level of protection. Although NO from activated macrophages can mediate cytotoxic killing of newly transformed schistoso...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·C J LowensteinS H Snyder
Dec 1, 1990·Infection and Immunity·F A LewisG M Niemann
May 1, 1988·The American Journal of Tropical Medicine and Hygiene·P S Coulson, R A Wilson
Jan 1, 1985·The American Journal of Tropical Medicine and Hygiene·F Von LichtenbergA Sher
Dec 1, 1974·Experimental Parasitology·F J Ramalho-PintoJ Pellegrino
Jul 1, 1983·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·D L Ennist, K H Jones
Feb 1, 1983·Experimental Parasitology·D A Dean
Feb 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·I P OswaldS L James
Nov 1, 1993·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·I M MacraeJ McCulloch
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R KamijoJ Vilcek

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Citations

Apr 11, 2000·Microscopy Research and Technique·D Rose, A Chiba
Nov 7, 2000·Parasitology Today·T A Wynn, K F Hoffmann
Feb 19, 2000·Current Opinion in Immunology·C BogdanA Diefenbach
Dec 22, 1999·Microbes and Infection·R A Wilson, P S Coulson
Oct 1, 2008·Infection and Immunity·Quentin D BickleHelena Helmby
Mar 17, 2006·The Journal of Parasitology·Gerry A P QuinnMarijke de Jong-Brink
Jun 28, 2001·Experimental Parasitology·L GanleyT V Rajan
Feb 20, 2010·Journal of Biomedicine & Biotechnology·Antonio Muro, José-Luís Pérez-Arellano
May 12, 2009·Molecular and Biochemical Parasitology·Xinzhi WangJiaojiao Lin
Dec 2, 2008·Molecular and Biochemical Parasitology·Takashi KumagaiTamotsu Kanazawa
Sep 6, 2005·Parasite Immunology·J P HewitsonA P Mountford
Jun 30, 2006·Molecular and Biochemical Parasitology·Takashi KumagaiTamotsu Kanazawa
Sep 12, 2006·Molecular and Biochemical Parasitology·Shanta M MesserliRobert M Greenberg
Aug 1, 1998·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·R A Wilson
Jun 26, 2020·Frontiers in Immunology·Jose Ma M AngelesPilarita T Rivera

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