PMID: 11929552Apr 4, 2002Paper

Nitric oxide production by a murine macrophage cell line (RAW264.7) stimulated with lipopolysaccharide from Actinobacillus actinomycetemcomitans

Oral Microbiology and Immunology
W SosrosenoHeni Susilowati

Abstract

The aim of this study was to determine whether Actinobacillus actinomycetemcomitans lipopolysaccharide (LPS-A. actinomycetemcomitans) could stimulate a murine macrophage cell line (RAW264.7 cells) to produce nitric oxide (NO). The cells were treated with LPS-A. actinomycetemcomitans or Escherichia coli LPS (LPS-Ec) for 24 h. The effects of N(G)-monomethyl-L-arginine (NMMA), polymyxin B and cytokines (IFN-gamma, TNF-alpha, IL-4 and IL-12) on the production of NO were also determined. The role of protein tyrosine kinase, protein kinase C and microtubulin organization on NO production were assessed by incubating RAW264.7 cells with genistein, bisindolylmaleide and colchicine prior to LPS-A. actinomycetemcomitans stimulation, respectively. NO levels from the culture supernatants were determined by the Griess reaction. The results showed that LPS-A. actinomycetemcomitans stimulated NO production by RAW264.7 cells in a dose-dependent manner, but was slightly less potent than LPS-Ec. NMMA and polymyxin B blocked the production of NO. IFN-gamma and IL-12 potentiated but IL-4 depressed NO production by LPS-A. actinomycetemcomitans-stimulated RAW264.7 cells. TNF-alpha had no effects on NO production. Genistein and bisindolylmalemaide, bu...Continue Reading

References

Oct 1, 1976·Immunochemistry·D C Morrison, D M Jacobs
Feb 1, 1992·Japanese Journal of Cancer Research : Gann·F MizuhashiI Tomita
Sep 1, 1994·The Journal of Experimental Medicine·E MartinQ W Xie
Jan 1, 1997·Microbiology·Ken-Ichi TanamotoToshio Umemoto
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Jun 1, 1997·Trends in Microbiology·D H Meyer, P M Fives-Taylor
Apr 4, 1998·European Journal of Oral Sciences·I J Blix, K Helgeland
Jan 8, 1999·Journal of Periodontal Research·M MatejkaH Sinzinger
Apr 15, 1999·International Journal of Radiation Biology·M RobertM Rouabhia
Feb 11, 2000·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·Y NemotoY Niho
Mar 17, 2000·Immunological Reviews·C BogdanA Diefenbach
Jun 8, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·S H BaekK B Kwun
Jul 6, 2000·Immunology Letters·J GołabM Jakóbisiak
Aug 10, 2000·Journal of Medical Microbiology·S KatoY Kowashi

❮ Previous
Next ❯

Citations

Sep 11, 2008·The Journal of Oral Implantology·Wihaskoro SosrosenoFikri Ibrahim
Feb 13, 2010·Journal of Clinical Periodontology·Hayana Ramos LimaAna Paula Campanelli
Jul 26, 2011·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Hang WangChang-Jer Wu
Jun 12, 2012·Experimental Cell Research·Yingting ZhuPeter Lance
May 13, 2018·The Journal of Immunology : Official Journal of the American Association of Immunologists·Andrew R DiNardoGeorge Makedonas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.