Nitric oxide production by peritoneal macrophages of cirrhotic rats: a host response against bacterial peritonitis

Gastroenterology
M Morales-RuizJ Rodés

Abstract

Patients and rats with cirrhosis and ascites are prone to develop peritonitis. The aim of this study was to assess whether peritoneal macrophages of cirrhotic rats without peritoneal infection produce nitric oxide and express inducible NO synthase (iNOS). NO2- accumulation produced by macrophages from control rats and cirrhotic rats with ascites was determined. iNOS messenger RNA and protein expression were analyzed by Northern and Western blot and immunocytochemical analysis. The in vivo effects of inhibiting iNOS were investigated by giving the specific iNOS inhibitor L-N-(1-iminoethyl)-lysine (L-NIL) or sterile saline to 9 and 7 cirrhotic rats with ascites, respectively. Cirrhotic macrophages produced NO2- that was around fourfold greater than that of control macrophages after 30 hours in culture. Northern and Western blot and immunocytochemical analysis showed the presence of iNOS messenger RNA and protein in macrophages of cirrhotic rats. Ascites cultures were positive in all rats administered L-NIL and negative in those administered saline. Macrophages of cirrhotic rats produce NO and express iNOS messenger RNA and protein, and these changes are not a consequence of overt bacterial infection. Because iNOS inhibition resul...Continue Reading

Citations

Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·G Garcia-TsaoG W Cadelina
Aug 26, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·W JiménezJ Rodés
Sep 18, 2012·Arab Journal of Gastroenterology : the Official Publication of the Pan-Arab Association of Gastroenterology·Mohammad M Abd ElaalGhada E Amr
Feb 22, 2005·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Reiner Wiest, Guadalupe Garcia-Tsao
Feb 14, 2007·Journal of Gastroenterology and Hepatology·Sathish Kumar NatarajanKunissery A Balasubramanian
Sep 22, 2001·Clinics in Liver Disease·V Shah
Jul 16, 1999·Journal of Hepatology·P SogniD Lebrec

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