Nitric oxide production from nitrite occurs primarily in tissues not in the blood: critical role of xanthine oxidase and aldehyde oxidase.

The Journal of Biological Chemistry
Haitao LiJay L Zweier

Abstract

Recent studies have shown that nitrite is an important storage form and source of NO in biological systems. Controversy remains, however, regarding whether NO formation from nitrite occurs primarily in tissues or in blood. Questions also remain regarding the mechanism, magnitude, and contributions of several alternative pathways of nitrite-dependent NO generation in biological systems. To characterize the mechanism and magnitude of NO generation from nitrite, electron paramagnetic resonance spectroscopy, chemiluminescence NO analyzer, and immunoassays of cGMP formation were performed. The addition of nitrite triggered a large amount of NO generation in tissues such as heart and liver, but only trace NO production in blood. Carbon monoxide increased NO release from blood, suggesting that hemoglobin acts to scavenge NO not to generate it. Administration of the xanthine oxidase (XO) inhibitor oxypurinol or aldehyde oxidase (AO) inhibitor raloxifene significantly decreased NO generation from nitrite in heart or liver. NO formation rates increased dramatically with decreasing pH or with decreased oxygen tension. Isolated enzyme studies further confirm that XO and AO, but not hemoglobin, are critical nitrite reductases. Overall, NO g...Continue Reading

References

Sep 1, 1987·Basic Research in Cardiology·M Muxfeldt, W Schaper
Oct 1, 1987·European Journal of Drug Metabolism and Pharmacokinetics·C BeedhamD J Rance
Oct 1, 1985·Archives of Biochemistry and Biophysics·S Yoshihara, K Tatsumi
Mar 1, 1984·Acta Pharmacologica Et Toxicologica·L A ShinobuM M Jones
Aug 1, 1995·Nature Medicine·J L ZweierP Kuppusamy
Aug 11, 1995·The Journal of Biological Chemistry·Y Xia, J L Zweier
Apr 7, 1994·Nature·N BenjaminH McKenzie
Apr 7, 1994·Nature·V E Buckwold
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·R M WrightJ E Repine
Dec 29, 1995·The Journal of Biological Chemistry·M L CalziE Garattini
Sep 1, 1996·The American Journal of Physiology·J R Wu-WongT J Opgenorth
Aug 29, 1998·Archives of Biochemistry and Biophysics·A SamouilovJ L Zweier
May 13, 1999·Biochimica Et Biophysica Acta·J L ZweierP Kuppusamy
Mar 14, 2000·The Journal of Biological Chemistry·B L GodberR Harrison
Mar 27, 2002·Archives of Biochemistry and Biophysics·Luisa Maia, Lurdes Mira
Jul 19, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mahesh S JoshiJack R Lancaster
Feb 22, 2003·Circulation Research·Sashwati RoyChandan K Sen
Sep 4, 2003·The Journal of Biological Chemistry·Enika NagababuJoseph M Rifkind
Sep 4, 2003·The Journal of Biological Chemistry·Sashwati RoyChandan K Sen
Nov 5, 2003·Nature Medicine·Kenyatta CosbyMark T Gladwin
Dec 4, 2003·Nature Medicine·David A Wink
Feb 10, 2004·The Journal of Biological Chemistry·Haitao LiJay L Zweier
Mar 22, 2005·American Journal of Respiratory Cell and Molecular Biology·John M Robinson, Jack R Lancaster
Apr 20, 2005·American Journal of Respiratory Cell and Molecular Biology·Mark T Gladwin
Apr 20, 2005·The Journal of Biological Chemistry·Kris T HuangNeil Hogg
Jul 26, 2005·The Journal of Clinical Investigation·Zhi HuangMark T Gladwin
Jan 13, 2006·Nature Chemical Biology·Mark T GladwinJon O Lundberg
Jan 31, 2006·Free Radical Biology & Medicine·Agnes KeszlerNeil Hogg
Feb 27, 2007·The Journal of Biological Chemistry·Rozalina GrubinaMark T Gladwin
Mar 14, 2007·Archives of Biochemistry and Biophysics·Tapan Kumar KunduJay L Zweier

❮ Previous
Next ❯

Citations

Jan 13, 2010·Analytical and Bioanalytical Chemistry·Mehjabin KathiwalaAnna Brajter-Toth
Nov 21, 2009·Journal of the American Chemical Society·Jun YiGeorge B Richter-Addo
Jun 30, 2011·Nature Communications·Takashi MiyamotoArdem Patapoutian
Feb 8, 2013·Nature Medicine·Louis H MillerThomas E Wellems
Oct 7, 2008·The Journal of Biological Chemistry·Martin FeelischHouman Ashrafian
Feb 7, 2012·Antioxidants & Redox Signaling·Nicoletta CastiglioneFrancesca Cutruzzolà
Nov 22, 2011·Antioxidants & Redox Signaling·Erin CurtisSruti Shiva
Jan 15, 2013·Antioxidants & Redox Signaling·Michele UmbrelloMervyn Singer
Oct 5, 2010·Cardiovascular Research·Julian O M OrmerodMichael P Frenneaux
Oct 19, 2010·Cardiovascular Research·Rakesh P PatelDaniel B Kim-Shapiro
Jul 19, 2013·European Heart Journal·Thomas MünzelTommaso Gori
Mar 26, 2010·Toxicological Sciences : an Official Journal of the Society of Toxicology·Heather B LeavesleyGary E Isom
Jun 12, 2010·Cardiology in Review·Vaughn E NossamanPhilip J Kadowitz
Oct 21, 2009·Current Opinion in Clinical Nutrition and Metabolic Care·Yvette C LuikingNicolaas E P Deutz
May 7, 2013·Annual Review of Nutrition·Eddie Weitzberg, Jon O Lundberg
Sep 27, 2012·American Journal of Physiology. Endocrinology and Metabolism·Yvette C LuikingNicolaas E P Deutz
Jun 10, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Wael F AlzawahraJay L Zweier
Dec 17, 2008·American Journal of Physiology. Heart and Circulatory Physiology·David B CaseyPhilip J Kadowitz
Nov 27, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Thomas E IngramPhilip E James
Jul 4, 2008·Blood·Mark T Gladwin, Daniel B Kim-Shapiro
Dec 27, 2011·Critical Reviews in Biomedical Engineering·Donald G BuerkDov Jaron
Jan 29, 2014·Chemical Reviews·Russ HillePartha Basu
Dec 10, 2013·Nitric Oxide : Biology and Chemistry·Daniel B Kim-Shapiro, Mark T Gladwin
Mar 22, 2014·Nitric Oxide : Biology and Chemistry·V KapilA Ahluwalia
Jul 6, 2014·Pharmacology & Therapeutics·J C BaileyM Madhani
May 31, 2011·Frontiers in Bioscience (Landmark Edition)·Leonid L Moroz, Andrea B Kohn
Dec 21, 2010·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Luisa B Maia, José J G Moura
Mar 25, 2011·Drug Metabolism Reviews·Enrico Garattini, Mineko Terao
Sep 4, 2015·Basic & Clinical Pharmacology & Toxicology·Victor Hugo Gonçalves-RizziCarlos A Dias-Junior
Oct 30, 2013·Clinical and Experimental Hypertension : CHE·Kunihiro SonodaJun Kobayashi
Jul 22, 2011·Critical Reviews in Biotechnology·Amit AgarwalU C Banerjee
Aug 21, 2013·Trends in Cardiovascular Medicine·Ulrike B Hendgen-CottaTienush Rassaf
Jan 2, 2010·Nitric Oxide : Biology and Chemistry·Jay L ZweierXiaoping Liu

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.