PMID: 9645394Jun 30, 1998Paper

Nitric oxide production in human macrophagic cells phagocytizing opsonized zymosan: direct characterization by measurement of the luminol dependent chemiluminescence

Free Radical Research
A GrossJ Dornand

Abstract

When differentiated into mature macrophages by the combination of all-trans retinoic acid and 1,25-dihydroxyvitamin D3, the human promonocytic cell lines U937 and THP-1 expressed inducible nitric oxide synthase (iNOS) transcripts. During their differentiation, the cells acquired the capacity to produce not only superoxide anion (O2.-) but also nitric oxide (.NO) in response to IgG (or IgE)-opsonized zymosan. The inhibitors of the iNOS pathway, aminoguanidine and NG-monomethyl-L-arginine (L-NMMA), suppressed the production of .NO and enhanced the steady-state concentration of O2.- determined. Conversely, superoxide dismutase (SOD) scavenged the O2.- released and increased the .NO-derived nitrite concentration detected. These data suggested a possible interaction between O2.- and .NO. In differentiated U937 (or THP-1) cells, IgG or IgE-opsonized zymosan induced a strong time-dependent luminol-dependent chemiluminescence (LDCL), which was abrogated by SOD and partially inhibited by aminoguanidine or L-NMMA. Since the iNOS inhibitors did not directly scavenge O2.-, LDCL determination in the presence or absence of SOD and/or iNOS inhibitors demonstrated a concomitant production of O2.- and .NO. These radicals induced the formation o...Continue Reading

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Citations

Dec 23, 2003·Microbial Pathogenesis·Antoine GrossJacques Dornand
Mar 25, 2004·Infection and Immunity·María P Jiménez de BagüésJacques Dornand
May 14, 2008·Journal of Endocrinological Investigation·J S QiJ H Liang
Jan 5, 2011·Journal of Immunological Methods·Margaret E AckermanGalit Alter
Aug 13, 2008·Journal of Ethnopharmacology·Yan-Ning LiJin-Sheng Qi
Jun 30, 2010·Luminescence : the Journal of Biological and Chemical Luminescence·Gorazd TompaPertti Marnila
Aug 29, 2000·Cephalalgia : an International Journal of Headache·G StirparoP Martelletti
Oct 16, 2002·Arteriosclerosis, Thrombosis, and Vascular Biology·Joseph J BoyleMartin R Bennett
Aug 13, 1999·The American Journal of Physiology·I Y HaddadB R Blazar

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