Nitric oxide production occurs after cytosolic alkalinization during stomatal closure induced by abscisic acid

Plant, Cell & Environment
Vijay K GonuguntaAgepati S Raghavendra

Abstract

Abscisic acid (ABA) raised the cytosolic pH and nitric oxide (NO) levels in guard cells while inducing stomatal closure in epidermis of Pisum sativum. Butyrate (a weak acid) reduced the cytosolic pH/NO production and prevented stomatal closure by ABA. Methylamine (a weak base) enhanced the cytosolic alkalinization and aggravated stomatal closure by ABA. The rise in guard cell pH because of ABA became noticeable after 6 min and peaked at 12 min, while NO production started at 9 min and peaked at 18 min. These results suggested that NO production was downstream of the rise in cytosolic pH. The ABA-induced increase in NO of guard cells and stomatal closure was prevented by 2-phenyl-4,4,5,5-tetramethyl imidazoline-1-oxyl 3-oxide (cPTIO, a NO scavenger) and partially by N-nitro-L-Arg-methyl ester (L-NAME, an inhibitor of NO synthase). In contrast, cPTIO or L-NAME had only a marginal effect on the pH rise induced by ABA. Ethylene glycol tetraacetic acid (EGTA, a calcium chelator) prevented ABA-induced stomatal closure while restricting cytosolic pH rise and NO production. We suggest that during ABA-induced stomatal closure, a rise in cytosolic pH is necessary for NO production. Calcium may act upstream of cytosolic alkalinization and...Continue Reading

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Citations

Mar 13, 2012·Journal of Experimental Botany·Kisten Sisse Krag GjettingAnja Thoe Fuglsang
Dec 25, 2009·Plant Physiology·Sarah M Assmann
Feb 12, 2013·The Plant Cell·Yuriko OsakabeKazuko Yamaguchi-Shinozaki
Jul 30, 2014·Nitric Oxide : Biology and Chemistry·Srinivas AgurlaAgepati S Raghavendra
Oct 29, 2019·PLoS Computational Biology·Parul MaheshwariReka Albert
Sep 10, 2020·Frontiers in Physiology·Parul MaheshwariReka Albert
Feb 11, 2015·Annual Review of Plant Biology·Yoshiyuki MurataShintaro Munemasa
Jan 12, 2010·Plant & Cell Physiology·Mohammad Mahbub IslamYoshiyuki Murata
Aug 27, 2010·Plant & Cell Physiology·Mohammad Mahbub IslamYoshiyuki Murata

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