Nitric oxide protects blood-brain barrier in vitro from hypoxia/reoxygenation-mediated injury

FEBS Letters
D I UtepbergenovI E Blasig

Abstract

A cell culture model of blood-brain barrier (BBB, coculture of rat brain endothelial cells with rat astrocytes) was used to investigate the effect of nitric oxide (.NO) on the damage of the BBB induced by hypoxia/reoxygenation (H/R). Permeability coefficient of fluorescein across the endothelium was used as a marker of BBB tightness. The permeability coefficient increased 5.2 times after H/R indicating strong disruption of the BBB. The presence of the .NO donor S-nitroso-N-acetylpenicillamine (SNAP, 30 microM), authentic .NO (6 microM) or superoxide dismutase (50 units/ml) during H/R attenuated H/R-induced increase in permeability. 30 microM SNAP or 6 microM .NO did not influence the function of BBB during normoxia, however, severe disruption was observed using 150 microM of SNAP and more than 24 microM of .NO. After H/R of endothelial cells, the content of malondialdehyde (MDA) increased 2.3 times indicating radical-induced peroxidation of membrane lipids. 30 microM SNAP or 6 microM authentic .NO completely prevented MDA formation. The results show that .NO may effectively scavenge reactive oxygen species formed during H/R of brain capillary endothelial cells, affording protection of BBB at the molecular and functional level.

References

Sep 1, 1989·The American Journal of Physiology·P G PhillipsM F Tsan
Jul 15, 1994·Biochemical and Biophysical Research Communications· Woldman YYuD I Utepbergenov
Jun 1, 1995·Annals of Medicine·R Busse, I Fleming
Aug 7, 1995·FEBS Letters·V Darley-UsmarB Halliwell
Apr 1, 1993·Journal of Cellular Physiology·O Durieu-TrautmannP O Couraud
Sep 1, 1994·Circulation Research·D JanigroH R Winn
Nov 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A WinkJ B Mitchell
Jan 5, 1996·The Journal of Biological Chemistry·A M MilesM B Grisham
Jan 1, 1995·Advances in Pharmacology·B A FreemanH Rubbo
May 1, 1996·Stroke; a Journal of Cerebral Circulation·W G Mayhan, S P Didion
Apr 1, 1996·Journal of Cellular Physiology·R D Hurst, I B Fritz
Jul 1, 1996·Chemical Research in Toxicology·H RubboB A Freeman
Oct 25, 1996·The Journal of Biological Chemistry·L E CrawfordP J Goldschmidt-Clermont

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Citations

Jun 21, 2005·Cellular and Molecular Neurobiology·Françoise Roux, Pierre-Olivier Couraud
Jun 21, 2005·Cellular and Molecular Neurobiology·Máiria A DeliMasami Niwa
Feb 3, 2012·Translational Stroke Research·Adam ChodobskiJoanna Szmydynger-Chodobska
Nov 14, 2003·Brain Research. Brain Research Protocols·Valentina V Jeliazkova-Mecheva, Dennis J Bobilya
Sep 23, 2000·Brain Research. Molecular Brain Research·K ChinC T Noguchi
Mar 6, 2002·British Journal of Pharmacology·Juan V Esplugues
Jan 18, 2011·Antioxidants & Redox Signaling·Ingolf E BlasigReiner F Haseloff
Apr 8, 2014·Journal of Neurosurgery·Bernardo Oliveira RatilalHelder Dias Mota-Filipe
Jan 5, 2001·Journal of Acquired Immune Deficiency Syndromes : JAIDS·M B Huang, V C Bond
Apr 27, 2010·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Alma RusMaria Angeles Peinado
Sep 23, 2009·Brain Research Reviews·Gwënaël PottiezYannis Karamanos
Sep 30, 2005·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·Oliver B DimitrijevicAnuska V Andjelkovic
Jun 7, 2008·Life Sciences·Christoph R W KuhlmannHeiko J Luhmann
Mar 28, 2013·Oxidative Medicine and Cellular Longevity·Ana-Maria EnciuBogdan O Popescu
May 4, 2011·Microscopy Research and Technique·Alma RusM Luisa Del Moral
Oct 21, 2016·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Li-Bing YeHong-Yu Zhang
Sep 12, 2014·PloS One·Catarina RapôsoMaria Alice da Cruz-Höfling
Feb 26, 2009·Free Radical Research·Pamela B L PunShabbir Moochhala
Jun 28, 2018·Journal of the American Heart Association·Juan Carlos Pena-PhilippidesTamara Roitbak
Oct 29, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·O O OgunsholaM Gassmann
Mar 30, 2006·American Journal of Physiology. Endocrinology and Metabolism·Robyn L O'KaneRichard A Hawkins

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