Nitric oxide reductase (norB) genes from pure cultures and environmental samples.

Applied and Environmental Microbiology
Gesche Braker, James M Tiedje

Abstract

A PCR-based approach was developed to recover nitric oxide (NO) reductase (norB) genes as a functional marker gene for denitrifying bacteria. norB database sequences grouped in two very distinct branches. One encodes the quinol-oxidizing single-subunit class (qNorB), while the other class is a cytochrome bc-type complex (cNorB). The latter oxidizes cytochrome c, and the gene is localized adjacent to norC. While both norB types occur in denitrifying strains, the qnorB type was also found in a variety of nondenitrifying strains, suggesting a function in detoxifying NO. Branch-specific degenerate primer sets detected the two norB types in our denitrifier cultures. Specificity was confirmed by sequence analysis of the norB amplicons and failure to amplify norB from nondenitrifying strains. These primer sets also specifically amplified norB from freshwater and marine sediments. Pairwise comparison of amplified norB sequences indicated minimum levels of amino acid identity of 43.9% for qnorB and 38% for cnorB. Phylogenetic analysis confirmed the existence of two classes of norB genes, which clustered according to the respective primer set. Within the qnorB cluster, the majority of genes from isolates and a few environmental clones fo...Continue Reading

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Citations

Jun 1, 2011·Environmental Science and Pollution Research International·Zhi-Feng ZhouJi-Zheng He
Jan 29, 2013·Applied Biochemistry and Biotechnology·Kathiravan Velusamy, Kishore Kumar Krishnani
Oct 15, 2013·Applied Biochemistry and Biotechnology·Yunlong YangFuqian Xu
Sep 24, 2011·Environmental Science & Technology·Kelly D FagerstoneAnthony J Marchese
Apr 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Takashi ObataHiroshi Kiyono
Apr 24, 2007·Applied and Environmental Microbiology·C E DandieC Goyer
Sep 30, 2008·Applied and Environmental Microbiology·Saleema Saleh-LakhaDavid L Burton
Aug 1, 2014·Water Research·Huijie LuDavid Stensel
Jan 28, 2016·Scientific Reports·Cintia Rodrigues MaruyamaRenata de Lima
Jun 5, 2012·Journal of Environmental Sciences (China)·Zhifeng ZhouJizheng He
Aug 3, 2010·Systematic and Applied Microbiology·Silke FalkGesche Braker
Oct 16, 2015·Environmental Science & Technology·Yunmeng PangGuodong Ji
Sep 11, 2004·Journal of Applied Microbiology·G Wang, H D Skipper
Oct 17, 2013·Bioresource Technology·Melissa P MezzariHugo M Soares
Mar 8, 2011·Microbial Biotechnology·Koki MaedaTakashi Osada
Mar 22, 2014·Environmental Microbiology·Satoshi IshiiSatoshi Okabe
Jan 27, 2011·Biochemical Society Transactions·Ines VerbaendertKim Heylen
Sep 2, 2014·TheScientificWorldJournal·Eiko E KuramaeJohannes A van Veen

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