Nitric oxide-releasing polyacrylonitrile disperses biofilms formed by wound-relevant pathogenic bacteria

Journal of Applied Microbiology
M CravenA D Strickland

Abstract

To test the antimicrobial and antibiofilm properties of a nitric oxide (NO)-releasing polymer against wound-relevant bacterial pathogens. Using a variety of 96-well plate assay systems that include standard well plates and the minimum biofilm eradication concentration biofilm assay well plate, a NO-releasing polymer based on (poly)acrylonitrile (PAN/NO) was studied for antimicrobial and antibiofilm activity against the common wound pathogens Pseudomonas aeruginosa (PAO1), Staphylococcus aureus (Mu50) and Enterococcus faecalis (V583). The polymer was capable of dispersing single-species biofilms of Ps. aeruginosa as well as a more clinically relevant multispecies biofilm that incorporates Ps. aeruginosa along with Staph. aureus and Ent. faecalis. PAN/NO also synergistically enhanced the susceptibility of the multispecies biofilms to the common broad-spectrum antibiotic, ciprofloxacin. Multiple in vitro biocompatibility assays show that PAN/NO has limited potential for mammalian cytotoxicity. This study demonstrates the feasibility of utilizing the NO-releasing polymer, PAN/NO, to manage biofilms formed by wound-relevant pathogens, and provides proof-of-concept for use of this NO-releasing polymer platform across multiple discipl...Continue Reading

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Citations

Apr 14, 2017·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Marie GominetDavid Lebeaux
Jun 2, 2017·Chemical Communications : Chem Comm·Kitty K K HoNaresh Kumar
Mar 14, 2020·British Journal of Nursing : BJN·Maria-Manuel AzevedoAcácio Rodrigues
Apr 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Amy XiaoRajamani Lakshminarayanan
May 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Zahra SadrearhamiCyrille Boyer
May 15, 2018·Advanced Healthcare Materials·Lei YangMark H Schoenfisch
Dec 11, 2020·British Journal of Community Nursing·Maria AzevedoAcácio Rodrigues
May 7, 2021·Chemical Communications : Chem Comm·Li WangXu Zhang

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