Nitric oxide signals postovulatory aging-induced abortive spontaneous egg activation in rats

Redox Report : Communications in Free Radical Research
Karuppanan V Premkumar, Shail K Chaube

Abstract

The aim of this study was to determine whether an increase of intracellular nitric oxide (NO) level signals postovulatory aging-induced abortive spontaneous egg activation (SEA) in rats. Freshly ovulated eggs (arrested at metaphase-II stage; M-II) were cultured in vitro for 3 hours to induce postovulatory egg aging. The morphological changes, inducible nitric oxide synthase (iNOS) expression, NO, cytosolic free Ca(2+), 3',5' cyclic guanosine monophosphate (cGMP), cell division cycle 25B (Cdc25B) and Wee1 levels, specific phosphorylation (pThr-14/Tyr-15) as well as total cyclin-dependent kinases-1 (Cdk1) (PSTAIRE) levels were analyzed. Postovulatory aging induced generation of NO possibly through an iNOS-mediated pathway. The increase in NO level was associated with augmented cytosolic free Ca(2+) as well as cGMP levels in aged eggs. A significant increase in Wee1 level and decrease of Cdc25B level were observed in aged eggs. An accumulation of phosphorylated Cdk1 (pThr-14/Tyr-15) level was observed in aged eggs, while total Cdk1 (PSTAIR) level remained unchanged. Our study demonstrates that generation of NO through an iNOS-mediated pathway increases cytosolic free Ca2+and cGMP levels. High levels of these signal molecules trigg...Continue Reading

References

Feb 1, 1991·Molecular Reproduction and Development·M Zernicka-Goetz
Dec 30, 1982·The Journal of Experimental Zoology·C L Keefer, A W Schuetz
Sep 23, 1994·Cell·H H Schmidt, U Walter
Mar 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·N G PublicoverK M Sanders
Dec 1, 1996·Current Opinion in Cell Biology·D J Lew, S Kornbluth
Feb 27, 1997·Nature·I WilmutK H Campbell
Mar 1, 2000·Molecular Reproduction and Development·A Jablonka-Shariff, L M Olson
Feb 22, 2001·Free Radical Biology & Medicine·R RadiA Cayota
Mar 26, 2002·Nature Genetics·A Jeannine LincolnPeter J Donovan
Jan 18, 2003·Reproduction : the Official Journal of the Society for the Study of Fertility·Rafael A FissoreJeremy Smyth
Jan 24, 2003·The Journal of Biological Chemistry·Calum LeckieMichael Whitaker
Sep 16, 2003·Molecular and Cellular Endocrinology·Shumin BuBo Zhou
Dec 11, 2003·Endocrine·Igor N Sergeev, Anthony W Norman
Jun 22, 2005·Molecular and Cellular Endocrinology·Jaroslav PetrFrantisek Jílek
Jul 23, 2005·IUBMB Life·Cecilia HidalgoM Angélica Carrasco
Sep 20, 2005·Current Biology : CB·Seung Jin HanMarco Conti
Dec 15, 2005·European Journal of Obstetrics, Gynecology, and Reproductive Biology·Qun LuZi-Jiang Chen
May 17, 2006·Zygote : the Biology of Gametes and Early Embryos·J PetrF Jílek
Jan 2, 2007·Cloning and Stem Cells·P J RossJ B Cibelli
Jun 16, 2007·Biochemical and Biophysical Research Communications·Jae Gyu Yoo, Lawrence C Smith
May 30, 2008·Frontiers in Bioscience : a Journal and Virtual Library·Songbo WangGuoling Xia
Jul 18, 2008·Biology of Reproduction·Cheng CuiBingzhi Yu
Apr 3, 2010·The Journal of Biological Chemistry·Oleg TimofeevIngrid Hoffmann
Sep 4, 2010·Antioxidants & Redox Signaling·Neelam Shahani, Akira Sawa
Jan 8, 2011·The Journal of Biological Chemistry·Jianying XiaoBingzhi Yu
Dec 8, 2011·The Journal of Biological Chemistry·Douglas T Hess, Jonathan S Stamler
Dec 15, 2012·Journal of Assisted Reproduction and Genetics·Karuppanan V Premkumar, Shail K Chaube
Apr 4, 2014·In Vitro Cellular & Developmental Biology. Animal·Karuppanan V Premkumar, Shail K Chaube

❮ Previous
Next ❯

Citations

Feb 21, 2016·In Vitro Cellular & Developmental Biology. Animal·Karuppanan V Premkumar, Shail K Chaube
Oct 5, 2016·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Shilpa Prasad, Shail K Chaube
Oct 30, 2016·Journal of Cellular Physiology·Meenakshi TiwariShail K Chaube
Apr 24, 2018·Journal of Biomedical Science·Pramod K YadavShail K Chaube
Aug 18, 2017·Development, Growth & Differentiation·Meenakshi Tiwari, Shail K Chaube
Jan 23, 2019·Journal of Biomedical Science·Govind R ChaudharyShail K Chaube
Feb 26, 2016·The Journal of Obstetrics and Gynaecology Research·Meenakshi Tiwari, Shail K Chaube
Dec 16, 2020·International Journal of Radiation Biology·Gozde Ozge OnderArzu Yay
Feb 12, 2021·Frontiers in Pharmacology·Liuqing YangQin Zhang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Cycle Pathways

Cell cycle is a complex process regulated by several signal transduction pathways and enzymes. Here is the latest research on regulation of cell cycle and cell cycle pathways.

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.