Nitric oxide suppression of human hematopoiesis in vitro. Contribution to inhibitory action of interferon-gamma and tumor necrosis factor-alpha

The Journal of Clinical Investigation
J P MaciejewskiN S Young

Abstract

IFN-gamma and TNF-alpha, potent inhibitors of hematopoiesis, induce nitric oxide synthase (NOS) in various cell types. When normal human bone marrow (BM) or CD34+ cells were exposed to NO, inhibition of colony formation was dose dependent and direct. NO induced apoptosis in BM progenitors, as shown by electrophoretic detection of DNA degradation and deoxynucleotidyl transferase assay. Using PCR and immunoprecipitation, we found inducible NOS (iNOS) mRNA and iNOS protein in BM after stimulation with IFN-gamma or TNF-alpha. iNOS mRNA was also detected by PCR in highly purified CD34+ cells; TNF-alpha or IFN-gamma increased iNOS expression. The presence of iNOS in CD34+ cells was confirmed in single cells by immunochemical staining. NG-Monomethyl-L-arginine (MM-Arg), an NOS inhibitor, partially reversed the effects of TNF-alpha and, to a lesser extent, IFN-gamma in methylcellulose culture of total BM and CD34+ cells, and inhibited apoptosis of BM cells induced by these cytokines. When the effects of competitive iNOS inhibition were tested on more immature progenitors, MM-Arg increased the number of long-term BM culture-initiating cells in control cultures but failed to protect these cells from the inhibitory action of IFN-gamma and...Continue Reading

References

Dec 18, 1992·Science·J S StamlerJ Loscalzo
Jan 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S StamlerJ Loscalzo
Sep 4, 1992·Cell·C J Lowenstein, S H Snyder
Jul 1, 1992·Acta Physiologica Scandinavica·S Moncada
Nov 21, 1992·Annals of the New York Academy of Sciences·P CeruttiP Amstad
Apr 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·T NguyenS R Tannenbaum
Feb 11, 1991·Current Opinion in Immunology·C F Nathan, J B Hibbs
Jun 1, 1990·Archives of Biochemistry and Biophysics·B Brüne, E G Lapetina
May 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·H J SutherlandC J Eaves
Jan 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·N C ZoumbosN S Young
Jun 14, 1967·Journal of Molecular Biology·B Hirt
Dec 30, 1993·The New England Journal of Medicine·S Moncada, A Higgs
Sep 1, 1994·The Journal of Experimental Medicine·E MartinQ W Xie
Mar 15, 1993·Biochemical and Biophysical Research Communications·M SarihA Adam
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
Aug 1, 1993·The Journal of Experimental Medicine·N McCartney-FrancisS M Wahl
Aug 18, 1993·Biochimica Et Biophysica Acta·H H SchmidtU Walter
Mar 30, 1994·Biochemical and Biophysical Research Communications·J RadonsH Kolb
Jan 1, 1994·Life Sciences·B BrüneE G Lapetina
Jan 1, 1993·Fundamental & Clinical Pharmacology·A Berdeaux
Mar 15, 1994·Annals of Internal Medicine·A Nisticò, N S Young
Jan 1, 1993·Archives of Biochemistry and Biophysics·D A WinkR W Nims

❮ Previous
Next ❯

Citations

Jan 29, 2000·Biulleten' eksperimental'noĭ biologii i meditsiny·Iu P Bel'skiĭS A Kusmartsev
May 31, 2003·International Journal of Hematology·Morag J Farquhar, David T Bowen
Nov 1, 1995·Research in Immunology·F Ouaaz
Jan 22, 2004·Archives of Medical Research·Jaroslaw P Maciejewski, Antonio Risitano
Nov 24, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·H J NaH M Kim
Dec 21, 2007·Nature Clinical Practice. Nephrology·Karien van der PuttenCarlo A J M Gaillard
Mar 20, 1999·Kidney International. Supplement·G Weiss
May 8, 1997·The New England Journal of Medicine·N S Young, J Maciejewski
Mar 11, 2005·The New England Journal of Medicine·Guenter Weiss, Lawrence T Goodnough
Jul 27, 1999·Surgery·W BarthlenJ Stadler
Jul 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jérémy FauconnierAlain Lacampagne
Oct 5, 2010·Journal of Tropical Pediatrics·Varun AggarwalSrikanta Basu
Nov 5, 1998·Journal of Clinical Gastroenterology·M TatemichiH Esumi
Feb 11, 2005·Journal of Pediatric Hematology/oncology·Artemis TsitsikaIoannis Papassotiriou
Jul 5, 2012·The Pediatric Infectious Disease Journal·Brian BergmarkWarren Zapol
Jul 13, 2004·Gut·C GascheG Weiss
Oct 13, 2006·Malaria Journal·Ian A ClarkWilliam B Cowden
Feb 7, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·R J van't Hof, S H Ralston
Dec 20, 2007·Molecular Medicine·Peter KrasnovGrigori Enikolopov
Aug 31, 2013·European Journal of Internal Medicine·Erika PoggialiIrene Motta
Apr 27, 2012·ISRN Hematology·Michael G Alexandrakis, George Tsirakis
Sep 23, 2014·BioMed Research International·Néstor Aarón Mosqueda-RomoMartha Legorreta-Herrera
Apr 29, 1998·Stem Cells·K SugiuraS Ikehara
Feb 26, 1998·Leukemia & Lymphoma·S RafiiM A Moore
Jan 29, 2013·Best Practice & Research. Clinical Anaesthesiology·Sumit SinghMitchell P Fink
Sep 22, 2010·Nitric Oxide : Biology and Chemistry·Melanie P Chin, William M Deen
Jul 28, 2009·Gastroenterología y hepatología·Felipe de la Morena López, Javier P Gisbert
Sep 13, 2008·Biochimica Et Biophysica Acta·Günter Weiss
Jul 28, 2007·Critical Reviews in Oncology/hematology·Vivien M HodgesA Peter Maxwell

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis