PMID: 9422945Jan 10, 1998Paper

Nitric oxide synthase activity and endothelial ultrastructure in ischaemic skin-flaps

British Journal of Plastic Surgery
O GribbeN P Wiklund

Abstract

The aim of the present study was to detect and quantify nitric oxide synthase (NOS) activity and to investigate morphological changes in the endothelium in two different ischaemic dorsal flap models in the rat, one based cranially and one based caudally. Intact skin from the dorsum was used as control. In both groups flaps were removed at 1, 4, 12, 24 and 72 h after surgery respectively. NOS-activity was measured by the conversion of L-arginine to L-citrulline and endothelial morphology was investigated using transmission electron microscopy. Intact skin showed Ca(2+)-dependent but no Ca(2+)-independent NOS-activity. A time-dependent decrease in Ca(2+)-dependent NOS-activity was seen in the proximal and distal part of the flaps in both flap models and was most pronounced in the distal part. Ca(2+)-independent NOS-activity increased in the proximal and distal part of flaps based cranially and in the proximal part of flaps based caudally. Morphological analysis of the endothelium showed signs of endothelial damage including blebbed membranes, swelling and endothelial loss. These findings show that ischaemia caused by skin-flap surgery leads to endothelial damage and a decrease in Ca(2+)-dependent nitric oxide synthase activity. F...Continue Reading

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Citations

Feb 13, 2003·Wound Repair and Regeneration : Official Publication of the Wound Healing Society [and] the European Tissue Repair Society·Rainer MittermayrHeinz Redl
Feb 13, 2007·Journal of Plastic, Reconstructive & Aesthetic Surgery : JPRAS·Orjan GribbeN P Wiklund
Dec 5, 2012·Acta Cirúrgica Brasileira·Jairo Zacchê de SáOlga Martins Marques
Jun 24, 2004·Annals of Plastic Surgery·Ewa Komorowska-TimekHarry J Buncke
May 29, 2004·Annals of Plastic Surgery·Feng ZhangWilliam C Lineaweaver
Jun 28, 2000·Current Opinion in Clinical Nutrition and Metabolic Care·D T EfronA Barbul
Jan 21, 2003·Archives of Facial Plastic Surgery·Babak AzizzadehKeith E Blackwell
Dec 19, 2019·Annals of Plastic Surgery·Emre GazyakanHolger Engel
Apr 9, 2001·Scandinavian Journal of Plastic and Reconstructive Surgery and Hand Surgery·K NakamuraM Itoman
Jan 29, 2019·Journal of Plastic Surgery and Hand Surgery·Bin ZhaoHede Yan
Sep 27, 2005·Journal of the American College of Surgeons·Shelby G ToppWilliam C Lineaweaver

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