Nitric oxide synthase activity in molluscan hemocytes

FEBS Letters
A Conte, E Ottaviani

Abstract

The hemocytes of the freshwater snail Viviparus ater have nitric oxide synthase (NOS) activity, as demonstrated by [3H]citrulline and nitrite + nitrate formation. The enzyme is NADPH dependent and is competitively inhibited by the mammalian NOS inhibitor NG-monomethyl-L-arginine (Ki = 4.7 microM). The Km for L-arginine is 2.5 microM. 70% of the total activity is observed at very low free Ca2+ concentration (3 nM). LPS treatment increased total NOS activity 2.4 fold. The activity is partly present in the non-soluble fraction of hemocytes (24% and 8% in non-stimulated and LPS-stimulated snails, respectively). An antiserum to the C-terminal synthetic pentadecapeptide of the rat cerebellar NOS inhibited the enzyme activity in a concentration-dependent manner. This is the first biochemical demonstration of the existence of NOS activity in molluscan hemocytes, the cells responsible for defence mechanisms.

References

Nov 15, 1992·The Biochemical Journal·P KlattB Mayer
Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·K McMillanB S Masters
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S LamasT Michel
Jul 28, 1992·Biochemistry·K A White, M A Marletta
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
Jan 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·H H SchmidtF Murad
Feb 1, 1991·Trends in Neurosciences·J Garthwaite
Dec 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J S PollockF Murad
Apr 28, 1989·Biochemical and Biophysical Research Communications·C D WrightH Osswald
Nov 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D S Bredt, S H Snyder
Jan 1, 1983·Developmental and Comparative Immunology·E Ottaviani
Oct 1, 1982·Analytical Biochemistry·L C GreenS R Tannenbaum
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·I G CharlesS Moncada
Apr 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·D A GellerT R Billiar
Mar 1, 1993·Neuroreport·R ElofssonD Sakharov

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Citations

Jan 15, 1999·Molecular Neurobiology·M Colasanti, G Venturini
Jan 1, 1995·Biology of the Cell·E OttavianiS Genedani
Jan 1, 1995·Advances in Neuroimmunology·A FranchiniE Ottaviani
Nov 4, 2000·The Science of the Total Environment·K L SmithM H Depledge
Feb 20, 2003·Developmental and Comparative Immunology·C TafallaA Figueras
Apr 8, 1998·Neuroscience Letters·A Conte, E Ottaviani
Jul 25, 2000·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·C E MorrallM H Depledge
Jul 1, 1997·General Pharmacology·D SonettiG B Stefano
Feb 24, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·E OttavianiA Franchini
May 29, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Carolina TafallaAntonio Figueras
Dec 23, 2003·The International Journal of Biochemistry & Cell Biology·Ana NovasJuan Ignacio Ramos-Martinez
Dec 8, 2005·Annual Review of Entomology·David Stanley
Jan 1, 1997·Annual Review of Immunology·J MacMickingC Nathan
Jun 10, 2006·International Journal of Biological Sciences·Laura GodoyRicard Albalat
Jun 15, 2010·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Eleni Banakou, Stefanos Dailianis
Jan 27, 2009·Developmental and Comparative Immunology·Erick García-GarcíaCarlos Rosales
Jun 19, 2007·Fish & Shellfish Immunology·Ana NovasJuan Ignacio Ramos-Martínez
Jul 7, 2007·Veterinary Immunology and Immunopathology·Catherine J WalshCharles A Manire
Mar 21, 2007·Developmental and Comparative Immunology·Luisa VillamilMarta Gómez-Chiarri
Dec 1, 2005·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Natraj KrishnanVladimír Simek
Jun 29, 2015·Fish & Shellfish Immunology·Maria G CastilloNina R Joffe
Jun 16, 1999·Nitric Oxide : Biology and Chemistry·J Weiske, A Wiesner
Jul 11, 2014·Fish & Shellfish Immunology·Xiao-Li YinZhi-Xun Guo
May 10, 2011·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Singaram GopalakrishnanKe-Jian Wang
Aug 17, 2000·Nitric Oxide : Biology and Chemistry·A J NappiY Carton
Feb 17, 2001·Nitric Oxide : Biology and Chemistry·I GourdonP Roch
Sep 20, 2007·Archives of Insect Biochemistry and Physiology·Lara J KohlerAnthony J Nappi
Mar 21, 2019·Frontiers in Physiology·Paula Mariela GonzálezGeorgina A Rivera-Ingraham
Mar 21, 2020·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Paula Mariela González, Susana Puntarulo
Sep 8, 2004·Insect Biochemistry and Molecular Biology·Antonia Herrera-OrtízMario H Rodríguez
Mar 7, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Feng-Ching YehChi-Ying Lee
Oct 14, 2008·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·M MonariG P Serrazanetti

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