Nitric oxide synthase immunoreactivity in the nematode Trichinella britovi. Evidence for nitric oxide production by the parasite

International Journal for Parasitology
M MasettiFabrizio Bruschi

Abstract

Nitric oxide has been extensively studied as an effector molecule of the host immune response against both protozoa and helminths, but parasites can also produce this molecule, through the action of nitric oxide (NO) synthases or NO synthases-like enzymes. The aim of this study was to verify the possible production of NO by Trichinella britovi L(1) larvae and the enzymes involved in this process. The NO synthase immunoreactivity and putative nitric oxide synthase-activity was analysed using antibodies to mammalian NO synthase III and to nitrotyrosine with immunohistochemistry, gold immunocytochemistry and immunoblot analysis and NADPH-diaphorase histochemistry. Our results show that T. britovi L(1) larvae possess an enzymatic activity capable of producing NO. The localisation of this activity, according to the NADPH-diaphorase histochemistry, is both at the cuticular and the internal level. This localisation is confirmed by nitrotyrosine immunohistochemistry both under optical and electron microscopy. Using the NO synthase III antibody, a similar pattern of labelling was found: in particular, electron microscopy showed a localisation of this immunoreactivity in the cuticle and in the stichocytes, where only the alpha2 granules ...Continue Reading

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Citations

Mar 2, 2010·Invertebrate Neuroscience : in·Robert J WalkerL Holden-Dye
Feb 20, 2010·Journal of Biomedicine & Biotechnology·Antonio Muro, José-Luís Pérez-Arellano
Jul 2, 2011·Parasitology International·Suksanti PrakobwongSomchai Pinlaor
Nov 9, 2006·Bioscience, Biotechnology, and Biochemistry·Qi ZhangHuilong Xiong
Oct 28, 2019·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Inese GavaraneMuza Kirjušina

Related Concepts

3-nitrotyrosine, (DL)-isomer
Antibodies, Protozoan
Immunocytochemistry
Tadpole
Electron Microscopy
NADPH Dehydrogenase
Nitric Oxide
Trichinella
Tyrosine
Western Blot

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