Nitric oxide synthase inhibitors decrease human polymorphonuclear leukocyte luminol-dependent chemiluminescence

Free Radical Biology & Medicine
S D CatzJ J Poderoso

Abstract

Nitric oxide synthase (NOS) inhibitors have been reported to modulate luminol-dependent chemiluminescence (CL) in rat macrophages, whereas the potent oxidant peroxynitrite (ONOO-) was shown to react with luminol to yield CL in a cell-free system. We evaluated the role of the L-arginine/NOS pathway in luminol CL by phorbol ester-activated human polymorphonuclear (PMN) leukocytes using the NOS inhibitors NG-monomethyl-L-arginine (L-NMMA) and N-iminoethyl-L-ornithine (L-NIO). Nitric oxide (.NO) release was determined by oxidation of oxymyoglobin. In addition, the effect of NOS inhibitors on superoxide anion O2.-) production was measured. Luminol CL was notably diminished by L-NMMA in a dose-dependent manner. Superoxide dismutase (SOD) also decreased luminol CL and L-NMMA potentiated light emission decrease produced by SOD. Nitric oxide and O2.- production was significantly decreased by L-NMMA; moreover, luminol-dependent CL but not O2.- production was attenuated by L-NIO. These data suggest that products of catalytic activity of both .NO synthase and NADPH oxidase are required to elicit maximal luminol CL in this system. These studies demonstrate that the NOS synthase pathway is involved in luminol CL by human PMN, and they sugges...Continue Reading

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Citations

Dec 8, 2000·Thrombosis Research·S Sethi, M Dikshit
Feb 22, 2001·Free Radical Biology & Medicine·R RadiA Cayota
Nov 5, 2008·Infection and Immunity·Melanie WellingtonDamian J Krysan
Aug 12, 2009·Analytica Chimica Acta·Marisa FreitasEduarda Fernandes
Oct 20, 2001·Redox Report : Communications in Free Radical Research·I WittmannJ Nagy
Oct 6, 2000·Nitric Oxide : Biology and Chemistry·E M GattoJ J Poderoso
Feb 17, 2001·Nitric Oxide : Biology and Chemistry·I GourdonP Roch
Jan 24, 2006·Neurotoxicology·Pablo GisoneSusana Puntarulo
Oct 13, 2006·Journal of Dairy Science·M RinaldiD D Bannerman
Sep 23, 1997·Journal of Cardiovascular Pharmacology·A T DemiryürekK A Kane
Jun 5, 2016·The Journal of Biological Chemistry·Cristina Álvarez-ZaldiernasArne Holmgren
Oct 4, 2000·Physiological Reviews·V Hampl, J Herget

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