Abstract
Nitric oxide mediates renal vasodilation and hyperfiltration during pregnancy in conscious rats through the endothelin B (ETB) receptor subtype. We tested the hypothesis that immunoreactive levels of endothelial nitric oxide synthase (eNOS) would be greater in the kidneys of midterm pregnant rats compared with virgin rats. We studied midterm pregnancy because renal plasma flow and glomerular filtration rate are maximal at this gestational stage. Western analysis was used to determine the level of eNOS in the three major zones of the kidney-inner medulla, outer medulla, and cortex-and in isolated small renal arteries, and in purified renal microvessels from the cortex. There were no significant differences in eNOS expression between virgin and midterm pregnant rats in any of those renal tissues, regardless of whether immunoreactivity was expressed as arbitrary densitometry units, as "microg placental equivalents" interpolated from the linear portion of a dose-response curve of placental villous protein (2.5-30 microg, positive control) run concurrently on each gel, or normalized for beta-actin. We also investigated other NOS isoforms. In particular, immunoreactive neuronal NOS (nNOS) was detectable in the inner and outer medulla...Continue Reading
References
Oct 1, 1992·Kidney International·P MundelW Kriz
Dec 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·C S WilcoxH H Schmidt
Jul 1, 1992·The Journal of Experimental Medicine·A K NusslerR L Simmons
Oct 25, 1990·Nature·D S BredtS H Snyder
Jul 1, 1984·Kidney International·K P Conrad
Sep 1, 1994·Kidney International·M G MohauptB C Kone
Nov 30, 1994·Biochemical and Biophysical Research Communications·R M GoetzJ Holtz
Jul 1, 1995·The Journal of Clinical Investigation·L A Danielson, K P Conrad
Oct 1, 1993·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K P ConradA K Davis
Mar 1, 1996·Hypertension·D L Mattson, D J Higgins
Jun 1, 1996·The American Journal of Physiology·I SinghJ P Briggs
Feb 1, 1997·The American Journal of Physiology·S M SladekK P Conrad
Feb 4, 1999·Hypertension·B T AlexanderJ P Granger
Feb 18, 1999·The Journal of Clinical Investigation·L A DanielsonK P Conrad
Mar 5, 1999·Brain Research·J M VanNessJ M Overton
Jan 21, 2000·Hypertension·D L Mattson, F Wu
Sep 27, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L A DanielsonK P Conrad
Dec 22, 2000·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·R E GandleyM K McLaughlin
Jun 8, 2001·The Journal of Clinical Investigation·J NovakK P Conrad
Jun 19, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·M KakokiD L Mattson
Oct 20, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·S R AbramJ P Granger
Mar 8, 2002·Hypertension·Barbara T AlexanderJoey P Granger
Jul 18, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Jacqueline NovakKirk P Conrad
Oct 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Yong Chool BooHanjoong Jo
Nov 1, 2003·Circulation Research·Arundhathi JeyabalanKirk P Conrad
Citations
Oct 6, 2011·Current Hypertension Reports·Kirk P Conrad, Sanjeev G Shroff
May 27, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kirk P Conrad
Jul 16, 2010·American Journal of Physiology. Renal Physiology·Cheryl A SmithChris Baylis
Nov 24, 2005·Clinical and Experimental Hypertension : CHE·Monica Ianosi-IrimieJules B Puschett
Jun 16, 2005·Annals of the New York Academy of Sciences·Kirk P ConradJacqueline Novak
Jul 24, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Kirk P Conrad, Jacqueline Novak
May 26, 2010·Hypertension·Kirk P Conrad
May 7, 2009·Annals of the New York Academy of Sciences·Jonathan T McGuaneKirk P Conrad
Oct 10, 2013·Journal of Cellular Physiology·Minghui OuRaouf A Khalil
Mar 22, 2014·American Journal of Physiology. Renal Physiology·Kirk P Conrad, John M Davison
Oct 22, 2016·American Journal of Physiology. Renal Physiology·Crystal A WestChris Baylis
Mar 12, 2005·Endocrinology·Laurie J KerchnerKirk P Conrad