PMID: 7542013Mar 1, 1995Paper

Nitric oxide synthase (NOS-I) in Leydig cells of the human testis

Archives of Histology and Cytology
Michail S DavidoffA F Holstein

Abstract

By means of immunocytochemical methods, immunoreactivity for the brain isoform of nitric oxide synthase (NOS-I) was recognized in numerous Leydig cells of the human testis as well as in MA-10 tumor and TM3 non-tumor mouse Leydig cell lines. Within the Leydig cell cytoplasm, immunocytochemical results suggested the occurrence of factors known to activate NOS-I such as glutamate and aspartate, as well as molecules involved in the regulation of the NOS-I activity such as calmodulin and Ca2+/calmodulin-dependent protein kinase II. Leydig cells, Sertoli cells, some endothelial cells of the testis, MA-10- and TM3 mouse Leydig cell lines exhibited a relatively strong NADPH-diaphorase enzyme activity as well. Double sequential immunostainings provided evidence that NOS-like immunoreactivity of the testicular Leydig cells is colocalized with testosterone, calmodulin, aspartate, glutamate, and Ca2+/calmodulin-dependent protein kinase II. Sodium nitro-prusside treatment did not result in increased cGMP formation by MA-10- or TM3 mouse Leydig cells, suggesting that NO produced by these cells acts primarily in a paracrine fashion. The NO produced by NOS-I immunoreactive Leydig cells may act as a messenger: 1) between neighbouring NOS-I posi...Continue Reading

Citations

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