Nitrile hydratase mediated green synthesis of lactamide by immobilizing Rhodococcus pyridinivorans NIT-36 cells on N, N'-Methylene bis-acrylamide activated chitosan.

International Journal of Biological Macromolecules
Poonam SinghAmit Seth

Abstract

In this paper green synthesis of an important commodity chemical lactamide has been undertaken using chitosan immobilized Rhodococcus pyridinivorans NIT-36 harbouring nitrile hydratase (NHase) enzyme. The cells immobilization (300 mg/g) is based on the partial entrapment of cells by suspension cross-linking technique facilitated by N, N'-Methylene bis-acrylamide. In the repeated-use experiments, the immobilized cells retained 80% of its initial activity when stored at 4 °C for 30 days. NHase activity of free and immobilized cells was studied over temperature ranging from 25 °C to 60 °C. The activity for free cells showed a sharp decline of 70% when the reaction temperature was elevated from 45 °C to 50 °C whereas chitosan immobilized cells retained their activity in the same temperature range. A fed-batch reaction was designed and the immobilized cells showed 100% similar enzymatic pattern for five consecutive rounds which gradually decreased in following cycles. A volumetric productivity of 20 g/L and catalytic productivity of 8.33 g/g dcw/h for lactamide were achieved.

Citations

Aug 27, 2021·ACS Applied Materials & Interfaces·Lu ChenQiang Zheng
Sep 27, 2021·Journal of Applied Microbiology·Ruchi SahuSavitha Janakiraman

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