Nitrite Control over Dissimilatory Nitrate/Nitrite Reduction Pathways in Shewanella loihica Strain PV-4

Applied and Environmental Microbiology
Sukhwan YoonFrank E Löffler

Abstract

Shewanella loihica strain PV-4 harbors both a functional denitrification (NO3 (-)→N2) and a respiratory ammonification (NO3 (-)→NH4 (+)) pathway. Batch and chemostat experiments revealed that NO2 (-) affects pathway selection and the formation of reduced products. Strain PV-4 cells grown with NO2 (-) as the sole electron acceptor produced exclusively NH4 (+). With NO3 (-) as the electron acceptor, denitrification predominated and N2O accounted for ∼90% of reduced products in the presence of acetylene. Chemostat experiments demonstrated that the NO2 (-):NO3 (-) ratio affected the distribution of reduced products, and respiratory ammonification dominated at high NO2 (-):NO3 (-) ratios, whereas low NO2 (-):NO3 (-) ratios favored denitrification. The NO2 (-):NO3 (-) ratios affected nirK transcript abundance, a measure of denitrification activity, in the chemostat experiments, and cells grown at a NO2 (-):NO3 (-) ratio of 3 had ∼37-fold fewer nirK transcripts per cell than cells grown with NO3 (-) as the sole electron acceptor. In contrast, the transcription of nrfA, implicated in NO2 (-)-to-NH4 (+) reduction, remained statistically unchanged under continuous cultivation conditions at NO2 (-):NO3 (-) ratios below 3. At NO2 (-):NO3 (...Continue Reading

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Feb 12, 2013·Applied and Environmental Microbiology·Sukhwan YoonFrank E Löffler

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Citations

Apr 17, 2016·Applied and Environmental Microbiology·Sukhwan YoonFrank E Löffler
May 13, 2017·AMB Express·Eveline M van den BergMark C M van Loosdrecht
Jul 21, 2017·Antonie van Leeuwenhoek·Yihua SunAnne Willems
Feb 7, 2019·Frontiers in Microbiology·David C VuonoJoseph J Grzymski
Mar 25, 2019·Journal of Microbiological Methods·Jordan CannonJoanne Chee-Sanford
Jun 17, 2020·The Science of the Total Environment·C B PandeyJ S Singh
Apr 6, 2021·Water Research·Ling-Dong ShiHe-Ping Zhao
May 25, 2021·Frontiers in Molecular Biosciences·Sylvain Durand, Maude Guillier
Apr 5, 2017·Environmental Science & Technology·Varun N Srinivasan, Caitlyn S Butler
Oct 24, 2020·Environmental Science & Technology·Mingsheng JiaEveline I P Volcke

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