Nitrite oxide and inducible nitric oxide synthase were regulated by polysaccharides isolated from Glycyrrhiza uralensis Fisch

Journal of Ethnopharmacology
Anwei ChengXueming Xu

Abstract

Water-soluble polysaccharide(Glycyrrhiza polysaccharide, GP) was isolated from Glycyrrhiza uralensis Fisch, with glycosidic units were composed of alpha (1-4) linked D-glucana. We demonstrated that GP significantly induces nitric oxides (NO) production and inducible NO synthase (iNOS) transcription in peritoneal macrophages. Moreover, iNOS mRNA expression was strongly induced by GP. NO in the culture supernatant was measured by Griess reaction, the production of iNOS was determined by commercially available iNOS kit. GP (10-400 microg/ml) alone increased significantly NO and iNOS production in macrophages. Macrophages simultaneously treated with GP plus lipopolysaccharide (LPS)/interferon-gamma (IFN-gamma) increased NO and iNOS production as compared to that of GP treatments alone. The production of NO and iNOS in macrophages pretreated with LPS followed by GP was higher than that of treatment with GP and LPS simultaneously. Using RT-PCR reveals that GP may provide a second triggering signal for the expression of iNOS mRNA. Thus, the iNOS-mediated NO synthesis in response to GP may be one of the mechanisms whereby this herbal medicine elicits its therapeutic effects.

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Citations

Apr 29, 2009·Journal of Natural Medicines·Abdel latif Shaker SeddekYasutake Shimizu
Apr 15, 2010·Zhong xi yi jie he xue bao = Journal of Chinese integrative medicine·Xiao-bing LiAi-ping Lu
Apr 4, 2016·Neurochemical Research·Yueling JinZhensheng Dai
Oct 4, 2015·International Journal of Biological Macromolecules·Liming LinXiaoming Wei
Jul 27, 2011·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Chunlin KeXiaoxiong Zeng
Aug 19, 2020·Journal of Ethnobiology and Ethnomedicine·Yong Xiong, Chunlin Long
Aug 15, 2017·Evidence-based Complementary and Alternative Medicine : ECAM·Yunfei JiXiaobo Li
Oct 2, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yi Shin EngJung San Chang
Apr 14, 2017·Medical Science Monitor : International Medical Journal of Experimental and Clinical Research·Jie ChenXin-Li Gu
Dec 12, 2021·The Journal of Pharmacy and Pharmacology·Yizhou LvYunpeng Fan

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