PMID: 9161026Apr 1, 1997Paper

Nitrite reductase expression is regulated at the post-transcriptional level by the nitrogen source in Nicotiana plumbaginifolia and Arabidopsis thaliana

The Plant Journal : for Cell and Molecular Biology
P CrétéC Meyer

Abstract

Higher plant nitrite reductase (NiR) is a monomeric chloroplastic protein catalysing the reduction of nitrite, the product of nitrate reduction, to ammonium. The expression of this enzyme is controlled at the transcriptional level by light and by the nitrogen source. In order to study the post-transcriptional regulation of NiR, Nicotiana plumbaginifolia and Arabidopsis thaliana were transformed with a chimaeric NiR construct containing the tobacco leaf NiR1 coding sequence driven by the CaMV 35S RNA promoter. Transformed plants did not show any phenotypic difference when compared with the wild-type, although they overexpressed NiR activity in the leaves. When these plants were grown in vitro on media containing either nitrate or ammonium as sole nitrogen source, NiR mRNA derived from transgene expression was constitutively expressed, whereas NiR activity and protein level were strongly reduced on ammonium-containing medium. These results suggest that, together with transcriptional control, post-transcriptional regulation by the nitrogen source is operating on NiR expression. This post-transcriptional regulation of tobacco leaf NiR1 expression was observed not only in the closely related species N. plumbaginifolia but also in th...Continue Reading

Citations

Aug 21, 2012·Applied Biochemistry and Biotechnology·Khalid Rehman HakeemMuhammad Iqbal
Dec 19, 2006·Plant Physiology·Lixing YuanNicolaus von Wirén
Jul 13, 2000·Plant Physiology·S D Lahiri, L A Allison
Jun 13, 2001·Plant Physiology·M TakahashiH Morikawa
May 5, 2001·Annual Review of Plant Physiology and Plant Molecular Biology·Arthur Grossman, Hideki Takahashi
Oct 9, 2015·Plant Physiology and Biochemistry : PPB·Susie DavenportJuan Pablo Sanchez-Tamburrrino
Jan 11, 2003·Biotechnology & Genetic Engineering Reviews·Don J Durzan, M Cristina Pedroso
Jun 16, 2010·The Plant Journal : for Cell and Molecular Biology·Wiebke ApelRalph Bock
Oct 1, 2015·Functional Plant Biology : FPB·Trevor GarnettMamoru Okamoto
Oct 30, 2020·The Plant Journal : for Cell and Molecular Biology·Erik M SolhaugClay J Carter
Jan 26, 2021·Plant Science : an International Journal of Experimental Plant Biology·Shahidul IslamWujun Ma
Mar 19, 2021·Frontiers in Plant Science·Samantha Vivia TheMechthild Tegeder

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