Nitrite reductase from Pseudomonas aeruginosa: sequence of the gene and the protein

FEBS Letters
M C SilvestriniM Brunori

Abstract

The gene coding for nitrite reductase of Pseudomonas aeruginosa has been cloned and its sequence determined. The coding region is 1707 bp long and contains information for a polypeptide chain of 568 amino acids. The sequence of the mature protein has been confirmed independently by extensive amino acid sequencing. The amino-terminus of the mature protein is located at Lys-26; the preceding 25 residue long extension shows the features typical of signal peptides. Therefore the enzyme is probably secreted into the periplasmic space. The mature protein is made of 543 amino acid residues and has a molecular mass of 60,204 Da. The c-heme-binding domain, which contains the only two Cys of the molecule, is located at the amino-terminal region. Analysis of the protein sequence in terms of hydrophobicity profile gives results consistent with the fact that the enzyme is fully water soluble and not membrane bound; the most hydrophilic region appears to correspond to the c-heme domain. Secondary structure predictions are in general agreement with previous analysis of circular dichroic data.

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Citations

Jan 1, 1993·Archives of Microbiology·W G Zumft
Apr 1, 1991·Journal of Bioenergetics and Biomembranes·A H Stouthamer
Feb 15, 1995·Journal of Inorganic Biochemistry·M C SilvestriniM Brunori
May 1, 1996·Journal of Inorganic Biochemistry·M C SilvestriniM Brunori
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Oct 3, 2002·Biochimica Et Biophysica Acta·Laurent Philippot
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Dec 3, 2014·International Journal of Medical Microbiology : IJMM·Mette KolpenPeter Østrup Jensen
Sep 13, 2019·Journal of Industrial Microbiology & Biotechnology·Gang ZhouQing-Shan Shi
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Apr 2, 1999·Applied and Environmental Microbiology·S Hallin, P E Lindgren
Jan 1, 1992·Applied and Environmental Microbiology·G B Smith, J M Tiedje

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