Nitrite/nitrate balance during photoinduced cerebral ischemia in the rat determined by high-performance liquid chromatography with UV and electrochemical detection

Journal of Chromatography. a
V RizzoR Moratti

Abstract

A specific and simple method for the direct simultaneous detection of extracellular nitrite (NO2-) and nitrate (NO3-) has been developed, using high-performance liquid chromatography separation with UV and electrochemical detection in series. These stable endproducts of nitric oxide (NO.) were determined in dialysis perfusate obtained through in vivo brain microdialysis during and after experimental photoinduced cerebral ischemia in rats. The chromatographic conditions were optimized with a reversed-phase column (250 x 46 mm) using 10 mM n-octylamine pH 6.0 as a mobile phase. Absorbance was measured at 220 nm for NO3- detection; electrochemical detection was performed at +0.7 V for NO2- evaluation. This assay system holds the advantages of in vivo consecutive measurements, high precision, good reproducibility, technical simplicity, fast response (about 7 min), and wide availability.

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Citations

Oct 26, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Camillo PortaLuciano Mutti
Dec 29, 2005·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Christine M WelchRichard G Compton
Nov 13, 2004·Biochimica Et Biophysica Acta·Yuling ZhaoHuibi Xu

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