PMID: 136Oct 1, 1975

Nitrofurazone-reducing enzymes in E. coli and their role in drug activation in vivo

Canadian Journal of Microbiology
D R McCallaM L Fan

Abstract

Earlier work showed that Escherichia coli contains at least two enzymes which reduce nitrofurazone and other nitrofuran derivatives. One of these enzymes is lacking in some nitrofurazone-resistant mutant strains. We now report that there are three separable nitrofuran reductases in this organism: reductase I (mol. wt. approximately 50 000, insensitive to O2), reductase IIa (mol. wt. approximately 120 000, inhibited by oxygen), reductase IIb (mol. wt. approximately 700 000, inhibited by O2). Unstable metabolites formed during the reduction of nitrofurazone by preparations containing reductases IIa and IIb produce breaks in DNA in vitro. In vivo experiments with nitrofurazone-resistant strains, which lack reductase II but contain reductases IIa and IIb, demonstrated that lethality, mutation, and DNA breakage are all greatly increased when cultures are incubated under anaerobic conditions, i.e., conditions such that reductase II is active. These results provide further evidence for the importance of reductive activation of nitrofurazone.

Citations

Jan 1, 1980·Naunyn-Schmiedeberg's Archives of Pharmacology·H G Jonen
Feb 1, 1977·Chemico-biological Interactions·P L Olive, D R McCalla
May 1, 1984·Chemico-biological Interactions·D W BryantB E McCarry
Feb 1, 1988·Mutation Research·J Velemínský, T Gichner
Nov 1, 1986·Mutation Research·E E Obaseiki-Ebor, J O Akerele
Jun 12, 2008·The Journal of Antimicrobial Chemotherapy·Linus SandegrenDan I Andersson
Dec 14, 2001·Journal of Bacteriology·E Suzanne PatersonI B Lambert
Aug 1, 1982·Toxicology and Applied Pharmacology·F J PetersonR P Mason
Apr 1, 1978·Chemico-biological Interactions·D R McCallaB Broughton
Aug 1, 1980·Chemico-biological Interactions·D W Bryant, D R McCalla
Jan 16, 1998·Human & Experimental Toxicology·C E BernardB Hoener
May 1, 1996·Human & Experimental Toxicology·W Shen, B Hoener
Jan 1, 1983·Environmental Mutagenesis·D R McCalla
May 1, 1988·American Journal of Hematology·M IsildarA A Yunis
Oct 22, 2019·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Nonkululeko H ZumaDavid D N'Da

Related Concepts

Aerobiosis
Anaerobiosis
Cell-Free System
DNA, Bacterial
Alkalescens-Dispar Group
Hydrogen-Ion Concentration
Alloenzymes
Nitrofurazone, Calcium (2: 1) Salt
RNA Denaturation
Oxidation-Reduction

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