Nitrogen- and sulfur-doped carbon dots as peroxidase mimetics: colorimetric determination of hydrogen peroxide and glutathione, and fluorimetric determination of lead(II)

Mikrochimica Acta
Mingyu TangZhongmin Su

Abstract

Fluorescent carbon dots co-doped with nitrogen and sulfur (N/S CDs) were prepared and found to display viable peroxidase mimicking activity. They have a blue fluorescence (with excitation/emission maxima at 340/456 nm) with a quantum yield of 35%. The N/S CDs catalyze the oxidation of 3,3,5,5-tetramethylbenzidine (TMB) in the presence of H2O2, and this leads to the appearance of a blue solution with a absorption maximum at 654 nm. A colorimetric method was developed for the determination of H2O2 that has a 1.75 μM detection limit and a linear response in the 10-5 to 10-4 M concentration range. The method can be extended to the enzymatic determination of glutathione with a 0.26 μM detection limit and a working range from 0.20 to 100 μM. In addition, the CDs respond to lead(II) which is a quencher of the blue fluorescence at 456 nm, with a detection limit of 11 μM and a working range up to 100 μM. Simultaneously, the color changes can be visually detected with absorbance signal changes from 10 to 100 μM with limit of 3.9 μM. A multiple detection system was worked out that allows monitoring of H2O2 and glutathione successively, and of lead(II). Graphical abstract (A) Schematic representation of the nitrogen & sulphur doped carbon ...Continue Reading

References

May 1, 2010·ACS Applied Materials & Interfaces·Fang ChaiZhongmin Su
May 9, 2012·Biosensors & Bioelectronics·Jingjing WangMeisheng Zhao
Aug 25, 2016·Chemistry : a European Journal·Chuanxi WangChi Zhang
Aug 16, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Zezhou YangXiaofeng Lu
Mar 30, 2018·Mikrochimica Acta·R R AnjanaGeorge Sony
Oct 13, 2018·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Zhenxing QinWeiping Xu
Jan 14, 2019·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Yan-Wen BaoFu-Gen Wu
Aug 28, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Vikas Kumar SinghSyed Hadi Hasan

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Citations

Oct 21, 2020·Analytical Methods : Advancing Methods and Applications·Burcu GökçalAli Tuncel
Jan 11, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Hai-Jiao WangQi-Qing Zhang
Jun 15, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Jingyu PangFang Chai

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