PMID: 9426594Jan 14, 1998Paper

Nitrogen availability and electron transport control the expression of glnB gene (encoding PII protein) in the cyanobacterium Synechocystis sp. PCC 6803

Plant Molecular Biology
M García-Domínguez, F J Florencio

Abstract

The glnB gene from Synechocystis sp. PCC 6803 that encodes the PII protein has been cloned by heterologous hybridization using the corresponding glnB gene from Synechococcus sp. PCC 7942. An ORF of 336 nucleotides appeared that potentially coded for a protein of 112 amino acid residues (M(r) 12,397). The deduced amino acid sequence revealed a high identity (higher than 80%) with its cyanobacterial counterparts and a basal level of identity (close to 60%) with other PII proteins. A single mRNA of about 680 nucleotides was found under all growth conditions studied. glnB gene expression was specifically activated under nitrogen deprivation (a 10-fold increase respect to nitrogen-replete conditions). No differences in glnB mRNA levels were observed when using nitrate or ammonium as nitrogen sources. Amount of glnB mRNA decreased to undetectable levels when transferring cells to the dark, but effect was avoided by adding glucose to the culture medium. Primer extension analysis and band-shift assays indicated that expression of the glnB gene, elevated under nitrogen deprivation, might lie under the control of the nitrogen transcriptional regulator NtcA, although constitutive levels of expression were also detected from a sigma 70-dep...Continue Reading

Citations

Dec 30, 1999·Journal of Bacteriology·M García-DomínguezF J Florencio
Oct 19, 2006·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Takashi OsanaiKan Tanaka
Sep 17, 2015·Journal of Basic Microbiology·Savita Singh, Arun Kumar Mishra
Jun 7, 2019·The New Phytologist·Rocío Robles-RengelM Isabel Muro-Pastor
Nov 13, 1998·Proceedings of the National Academy of Sciences of the United States of America·M H HsiehG Coruzzi
Feb 29, 2000·Journal of Bacteriology·M García-DomínguezJ C Reyes
Nov 1, 2001·Proceedings of the National Academy of Sciences of the United States of America·A Irmler, K Forchhammer
Sep 7, 2005·Photosynthesis Research·M Isabel Muro-PastorFrancisco J Florencio
Mar 13, 2018·Critical Reviews in Microbiology·Alberto A Esteves-FerreiraRonan Sulpice
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·M García-DomínguezF J Florencio
Jan 3, 2001·Journal of Bacteriology·A HerreroE Flores
Oct 4, 2006·Molecular Systems Biology·Andrew C TolonenSallie W Chisholm
Oct 20, 2009·Gene Regulation and Systems Biology·Sousuke Imamura, Munehiko Asayama
Dec 19, 2008·Science in China. Series C, Life Sciences·Ying Zhang, JinDong Zhao
Dec 30, 2014·MicrobiologyOpen·Joaquín Giner-LamiaFrancisco J Florencio
Mar 10, 2001·Microbiology and Molecular Biology Reviews : MMBR·T ArcondéguyM Merrick
Mar 26, 2002·Journal of Experimental Botany·Michael Hodges
Mar 23, 2017·Genetics and Molecular Biology·Alberto A Esteves-FerreiraWagner L Araújo
Apr 4, 2002·Molecular Microbiology·Ana ValladaresEnrique Flores
May 7, 2014·PloS One·Ana María Sánchez-RiegoFrancisco Javier Florencio

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