Nitrogen doped carbon dots derived from Sargassum fluitans as fluorophore for DNA detection

Journal of Photochemistry and Photobiology. B, Biology
S GodavarthiL Martinez Gomez

Abstract

This work focused on the use of waste seaweed Sargassum fluitans (S. fluitans) as carbon source precursor to prepare nitrogen doped carbon dots (NCDs) by hydrothermal method. High resolution transmission electron microscopic (HR-TEM) studies revealed that the synthesized water soluble NCDs are in the size range of 2-8nm and exhibits excellent fluorescent properties with a quantum yield of 18.2%. Elemental nitrogen in NCDs was evidenced by X-ray photoelectron spectroscopy (XPS) and Fourier transformed infrared spectrum (FT-IR). The phytochemical analysis of S. fluitans using (1)H NMR and (13)C NMR revealed the presence of few amino acids which act as nitrogen source in the preparation of NCDs. Application of NCDs as fluorophore for double stranded DNA, single stranded DNA and RNA detection was highlighted in this study. Excellent fluorescent tagging abilities of NCDs with the biological nucleic acids were evidenced using gel electrophoresis. Significant increase in fluorescence was observed upon tagging of NCDs with nucleic acids and this particular phenomenon helps better in visualizing the nucleic acids. All three nucleic acids i.e. double stranded DNA, single stranded DNA and RNA showed similar phenomenon upon tagging with NC...Continue Reading

Citations

Jun 21, 2020·Biosensors·Muhammad ZulfajriGenin Gary Huang
Nov 25, 2017·Chemical Record : an Official Publication of the Chemical Society of Japan ... [et Al.]·Hui Feng, Zhaosheng Qian
Apr 28, 2018·Marine Drugs·Anna Ilnicka, Jerzy P Lukaszewicz
Apr 3, 2020·Current Pharmaceutical Design·Eemaan N CohenViness Pillay
Nov 27, 2020·Nanomaterials·Chao KangZeng Ping Chen
Apr 20, 2021·Colloids and Surfaces. B, Biointerfaces·Legha AnsariMohammad Amjadi
May 14, 2021·Luminescence : the Journal of Biological and Chemical Luminescence·Nurul Amalia HumaeraDahlang Tahir

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