Nitrogen metabolism, acid-base regulation, and molecular responses to ammonia and acid infusions in the spiny dogfish shark (Squalus acanthias)

Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology
C Michele NawataChris M Wood

Abstract

Although they are ureotelic, marine elasmobranchs express Rh glycoproteins, putative ammonia channels. To address questions raised by a recent study on high environmental ammonia (HEA) exposure, dogfish were intravascularly infused for 24 h at 3 ml kg(-1) h(-1) with isosmotic NaCl (500 mmol l(-1), control), NH4HCO3 (500 mmol l(-1)), NH4Cl (500 mmol l(-1)), or HCl (as 125 mmol l(-1) HCl + 375 mmol l(-1) NaCl). While NaCl had no effect on arterial acid-base status, NH4HCO3 caused mild alkalosis, NH4Cl caused strong acidosis, and HCl caused lesser acidosis, all predominantly metabolic in nature. Total plasma ammonia (T(Amm)) and excretion rates of ammonia (J(Amm)) and urea-N (J(Urea-N)) were unaffected by NaCl or HCl. However, despite equal loading rates, plasma T(Amm) increased to a greater extent with NH4Cl, while J(Amm) increased to a greater extent with NH4HCO3 due to much greater increases in blood-to-water PNH3 gradients. As with HEA, both treatments caused large (90%) elevations of J(Urea-N), indicating that urea-N synthesis by the ornithine-urea cycle (OUC) is driven primarily by ammonia rather than HCO3(-). Branchial mRNA expressions of Rhbg and Rhp2 were unaffected by NH4HCO3 or NH4Cl, but v-type H(+)-ATPase was down-reg...Continue Reading

References

May 15, 1985·Archives of Biochemistry and Biophysics·R A Shankar, P M Anderson
Jun 1, 1981·Biulleten' eksperimental'noĭ biologii i meditsiny·L V Filippova
Oct 23, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Rahmatullah, T R Boyde
Mar 20, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·P PärtC M Wood
Jan 31, 2002·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·S L EdwardsC-M Tse
Sep 14, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Peter M PiermariniDavid H Evans
Dec 20, 2003·Journal of Comparative Physiology. B, Biochemical, Systemic, and Environmental Physiology·A R Cooper, S Morris
Feb 6, 2004·The Journal of Biological Chemistry·Naziha BakouhGabrielle Planelles
Apr 21, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric SoupeneSydney Kustu
Dec 2, 2004·Proceedings of the National Academy of Sciences of the United States of America·Pierre RipocheJean-Pierre Cartron
Dec 8, 2004·The Journal of Experimental Biology·Dirk WeihrauchDavid W Towle
Jul 5, 2005·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Keith P ChoeDavid H Evans
Jul 8, 2005·The Journal of Experimental Biology·Chris M WoodPatrick J Walsh
Oct 18, 2005·Proceedings of the National Academy of Sciences of the United States of America·Cheng-Han Huang, Jianbin Peng
Mar 28, 2006·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·S Kustu, W Inwood
Mar 28, 2006·Transfusion clinique et biologique : journal de la Société française de transfusion sanguine·V EndewardG Gros
Oct 20, 2006·Respiratory Physiology & Neurobiology·Chris M WoodTrevor J Shuttleworth
Jan 6, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Martin TresguerresGreg G Goss
Jan 16, 2007·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Tsutomu NakadaShigehisa Hirose
Jul 28, 2007·Respiratory Physiology & Neurobiology·Chris M WoodSunita Nadella
Dec 21, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·James B ClaiborneSusan L Edwards
Apr 19, 2008·Physiological and Biochemical Zoology : PBZ·Chris M WoodPatrick J Walsh
Oct 9, 2008·The Journal of Experimental Biology·C Michele Nawata, Chris M Wood
Jul 22, 2009·Respiratory Physiology & Neurobiology·Li Zhang, Chris M Wood
Feb 4, 2010·PloS One·Isabelle Mouro-ChanteloupPierre Ripoche
Aug 5, 2010·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Steve F PerryPatrick J Walsh
Aug 13, 2011·The Journal of Experimental Biology·Beau D ReillyCraig E Franklin
Sep 14, 2011·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·W Gary AndersonDirk Weihrauch
Nov 22, 2011·Journal of Insect Physiology·Dirk WeihrauchMichael J O'Donnell
Jul 23, 2013·The Journal of Experimental Biology·Li ZhangChris M Wood
Dec 3, 2014·Respiratory Physiology & Neurobiology·Gudrun De Boeck, Chris M Wood
Dec 4, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Li ZhangChris M Wood
Dec 19, 2014·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Zhongyan LuZanming Shen

❮ Previous
Next ❯

Citations

Feb 13, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Samuel C Guffey, Greg G Goss
Jul 28, 2007·Respiratory Physiology & Neurobiology·Chris M WoodSunita Nadella

❮ Previous
Next ❯

Related Concepts

Related Feeds

ATP Synthases

ATP synthases are enzymes located in the inner mitochondrial membrane that catalyze the synthesis of ATP during cellular respiration. Discover the latest research on ATP synthases here.