Nitrogen or sulfur starvation differentially affects phycobilisome degradation and expression of the nblA gene in Synechocystis strain PCC 6803

Journal of Bacteriology
Catherine RichaudJean-Claude Thomas

Abstract

Nitrogen (N) limitation in cyanobacteria is well documented: a reduced growth rate is observed, accompanied by a cessation of phycobiliprotein synthesis and an ordered degradation of phycobilisomes (PBS). This leads to a dramatic bleaching phenomenon known as chlorosis. In Synechococcus strain PCC 7942, bleaching due to PBS degradation is also observed under sulfur (S) or phosphorus (P) limitation, and all three are under the control of the nblA gene product, a 59-amino-acid polypeptide which is overexpressed under N, S, and P starvation (J. L. Collier, and A. R. Grossman, EMBO J. 13:1039-1047, 1994). Cyanobase sequence data for Synechocystis strain PCC 6803 indicate the presence of two tandem open reading frames (sll0452 and sll0453) homologous to nblA. We cloned the two genes, identified a unique 5' mRNA end suggestive of a single transcription start site, and studied nblA expression under conditions of N or S starvation by Northern hybridization: transcripts were detected only under N starvation (no signal is detected in replete medium or with S starvation), whether nblA1 or nblA2 was used as a probe. Mutations in nblA1 and nblA2 were constructed by insertion of a kanamycin cassette; both mutations were nonbleaching under N ...Continue Reading

References

Nov 18, 1977·Archives of Microbiology·L S Miller, S C Holt
Jan 1, 1975·Archives of Microbiology·M J Ihlenfeldt, J Gibson
Jan 1, 1969·Archiv für Mikrobiologie·M M Allen, A J Smith
Oct 1, 1983·Journal of Bacteriology·D M Sherman, L A Sherman
Mar 1, 1980·Journal of Bacteriology·N B Wood, R Haselkorn
Aug 31, 1995·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·T KanekoS Tabata
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·C Richaud, G Zabulon
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·R Schwarz, A R Grossman
Jan 16, 1999·The Journal of Biological Chemistry·J C Thomas, C Passaquet
Dec 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·R P LegockiA A Szalay

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Citations

Jun 2, 2007·Archives of Microbiology·Waraporn JuntarajumnongJulian J Eaton-Rye
Dec 16, 2006·Current Microbiology·Gérald ZabulonJean-Claude Thomas
Jun 6, 2003·Trends in Biotechnology·Till Bachmann
Jun 7, 2013·The ISME Journal·Lara RajeevAindrila Mukhopadhyay
Jun 9, 2006·Microbiology and Molecular Biology Reviews : MMBR·Mark K Ashby, Jean Houmard
Jul 23, 2008·BMC Genomics·Zhigang ZhangArkady Khodursky
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Jean-Claude ThomasGhada Ajlani
May 7, 2014·The Plant Journal : for Cell and Molecular Biology·Eleonora SenderskyRakefet Schwarz
Jul 16, 2015·The Plant Journal : for Cell and Molecular Biology·Eleonora SenderskyRakefet Schwarz
Mar 22, 2014·Microbiology·Frédéric DeschoenmaekerRuddy Wattiez
May 12, 2016·Photosynthesis Research·Sindhujaa VajravelTunde N Toth
Aug 17, 2016·Biochimica Et Biophysica Acta·Long-Sheng ZhaoYu-Zhong Zhang
Oct 23, 2016·Journal of Biotechnology·Katsunori NamakoshiHiroshi Shimizu
Mar 12, 2016·PloS One·Andrés GonzálezMaría F Fillat
Sep 4, 2015·Integrative Biology : Quantitative Biosciences From Nano to Macro·Jiangxin WangWeiwen Zhang
Jul 25, 2009·Bulletin of Environmental Contamination and Toxicology· Hemlata, Tasneem Fatma
Oct 16, 2018·Environmental Microbiology·Karl Forchhammer, Rakefet Schwarz
Mar 24, 2020·Nature Microbiology·Kristin A MooreJeffrey C Cameron
May 25, 2011·Lab on a Chip·Xu ShiDeirdre R Meldrum
May 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Yuma TokumaruHiroshi Shimizu

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