PMID: 6113591Apr 1, 1981Paper

Nitrogen regulatory locus "glnR" of enteric bacteria is composed of cistrons ntrB and ntrC: identification of their protein products

Proceedings of the National Academy of Sciences of the United States of America
N McFarlandS Kustu

Abstract

The nitrogen regulatory locus "glnR" of Escherichia coli and Salmonella typhimurium is composed of two cistrons, which we propose to call ntrB and ntrC (nitrogen regulation B and C). Frameshift mutations in ntrB and ntrC were isolated on a lambda phage that carries the E. coli ntrB and ntrC genes and the closely linked glnA gene, the structural gene encoding glutamine synthetase [L-glutamate:ammonia ligase (ADP-forming), EC 6.3.1.2]; mutations were selected as suppressors of glnF (which we propose to rename ntrA), a selection used previously to isolate glnR mutations. Phage DNA from one mutant (ntrB) failed to direct synthesis of a 36-kilodalton (kDal) protein whose synthesis was directed by DNA from the parent phage (ntrB+) in a coupled in vitro transcription/translation system. DNA from three other mutants (ntrC) failed to direct synthesis of a 54-kDal protein; DNA from two of these mutants instead directed synthesis of smaller proteins, 53 and 50 kDal, respectively. In all four cases, DNA from frameshift revertants directed synthesis of both the 36-kDal and 54-kDal proteins. These results suggested that ntrB and ntrC were separate genes which encoded 36-kDal and 54-kDal protein products, respectively. Frameshift mutations in...Continue Reading

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Citations

Jan 1, 1981·Molecular & General Genetics : MGG·G R Wei, S Kustu
Jan 1, 1983·Molecular & General Genetics : MGG·A A Covarrubias, F Bastarrachea
Jan 1, 1982·Molecular & General Genetics : MGG·T MacNeilB Tyler
Jan 1, 1984·Molecular & General Genetics : MGG·I Castaño, F Bastarrachea
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Jan 1, 1983·Biotechnology Advances·R Hütter, P Niederberger
Nov 17, 2010·Proceedings of the National Academy of Sciences of the United States of America·TuAnh Ngoc HuynhValley Stewart
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·C F Higgins, G F Ames
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·V SundaresanF M Ausubel
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·L J Reitzer, B Magasanik
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J HirschmanS Kustu
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