Nitrogen-responsive regulation of GATA protein family activators Gln3 and Gat1 occurs by two distinct pathways, one inhibited by rapamycin and the other by methionine sulfoximine.

The Journal of Biological Chemistry
Isabelle GeorisEvelyne Dubois


Nitrogen availability regulates the transcription of genes required to degrade non-preferentially utilized nitrogen sources by governing the localization and function of transcription activators, Gln3 and Gat1. TorC1 inhibitor, rapamycin (Rap), and glutamine synthetase inhibitor, methionine sulfoximine (Msx), elicit responses grossly similar to those of limiting nitrogen, implicating both glutamine synthesis and TorC1 in the regulation of Gln3 and Gat1. To better understand this regulation, we compared Msx- versus Rap-elicited Gln3 and Gat1 localization, their DNA binding, nitrogen catabolite repression-sensitive gene expression, and the TorC1 pathway phosphatase requirements for these responses. Using this information we queried whether Rap and Msx inhibit sequential steps in a single, linear cascade connecting glutamine availability to Gln3 and Gat1 control as currently accepted or alternatively inhibit steps in two distinct parallel pathways. We find that Rap most strongly elicits nuclear Gat1 localization and expression of genes whose transcription is most Gat1-dependent. Msx, on the other hand, elicits nuclear Gln3 but not Gat1 localization and expression of genes that are most Gln3-dependent. Importantly, Rap-elicited nuc...Continue Reading


Dec 1, 1979·Journal of Bacteriology·V Turoscy, Terrance G Cooper
Jan 1, 1985·Advances in Microbial Physiology·J M WiameH N Arst
Feb 1, 1988·Journal of Bacteriology·W E Courchesne, Boris Magasanik
Aug 1, 1987·Journal of Bacteriology·Rajendra RaiT G Cooper
Jul 17, 1973·Biochemical and Biophysical Research Communications·R DrillienF Lacroute
Jan 1, 1972·Journal of Bacteriology·R Drillien, F Lacroute
Dec 1, 1984·Molecular and Cellular Biology·A P Mitchell, Boris Magasanik
Oct 10, 1995·Proceedings of the National Academy of Sciences of the United States of America·M StanbroughBoris Magasanik
Nov 11, 1999·Molecular Biotechnology·J Hofman-Bang
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·J S HardwickS L Schreiber
Jan 5, 2000·Genes & Development·M E CardenasJ Heitman
Aug 15, 2000·The Journal of Biological Chemistry·P G BertramX F Steven Zheng
May 2, 2001·The Journal of Biological Chemistry·John CarvalhoX F Steven Zheng
Jun 16, 2001·The Journal of Biological Chemistry·Ajit KulkarniT G Cooper
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·José L CrespoMichael N Hall
Jun 14, 2002·Gene·Boris Magasanik, Chris A Kaiser
Mar 8, 2003·The Journal of Biological Chemistry·John Carvalho, X F Steven Zheng
Oct 11, 2003·Molecular Cell·Zhengchang LiuRonald A Butow
Oct 11, 2003·Molecular Biology of the Cell·Huamin WangYu Jiang
Oct 29, 2005·The Journal of Biological Chemistry·Sergio GiannattasioRonald A Butow
Apr 19, 2007·The Journal of Biological Chemistry·Jennifer J Tate, Terrance G Cooper
Feb 29, 2008·Annual Review of Genetics·Shadia ZamanJames R Broach


Oct 1, 2015·Molecular Microbiology·Mohammad Fayyad-KazanIsabelle Georis
Feb 4, 2014·FEMS Microbiology Reviews·Michaela ConradJohan M Thevelein
May 24, 2012·Cell Cycle·Martin Kupiec, Ronit Weisman
Mar 12, 2016·PLoS Computational Biology·Andreas Milias-ArgeitisJohn Lygeros
Jul 4, 2017·Biomolecules·Yoko OtsuboAkira Yamashita
Feb 14, 2018·Microbiology and Molecular Biology Reviews : MMBR·Weiping ZhangJian Chen
Dec 11, 2013·The Journal of Biological Chemistry·Rajendra RaiTerrance G Cooper
Jun 21, 2017·Antonie van Leeuwenhoek·Danielli Batista Bezerra CajueiroWill de Barros Pita
Jul 5, 2017·Molecular and Cellular Biology·Jonathan Merran, Jeffry L Corden
May 31, 2015·G3 : Genes - Genomes - Genetics·Jennifer J TateTerrance G Cooper
May 23, 2014·The Journal of Biological Chemistry·Rajendra RaiTerrance G Cooper

Related Concepts

GLN3 protein, S cerevisiae
GAT1 protein, S cerevisiae
TORC1 protein complex, S cerevisiae
Antibiotics, Antifungal
Cell Nucleus
Methionine Sulfoximine
Saccharomyces cerevisiae
Transcription Factor
Gene Expression Regulation, Fungal

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