Nitrogen Starvation Acclimation in Synechococcus elongatus: Redox-Control and the Role of Nitrate Reduction as an Electron Sink

Life
Alexander KlotzKarl Forchhammer

Abstract

Nitrogen starvation acclimation in non-diazotrophic cyanobacteria is characterized by a process termed chlorosis, where the light harvesting pigments are degraded and the cells gradually tune down photosynthetic and metabolic activities. The chlorosis response is governed by a complex and poorly understood regulatory network, which converges at the expression of the nblA gene, the triggering factor for phycobiliprotein degradation. This study established a method that allows uncoupling metabolic and redox-signals involved in nitrogen-starvation acclimation. Inhibition of glutamine synthetase (GS) by a precise dosage of l-methionine-sulfoximine (MSX) mimics the metabolic situation of nitrogen starvation. Addition of nitrate to such MSX-inhibited cells eliminates the associated redox-stress by enabling electron flow towards nitrate/nitrite reduction and thereby, prevents the induction of nblA expression and the associated chlorosis response. This study demonstrates that nitrogen starvation is perceived not only through metabolic signals, but requires a redox signal indicating over-reduction of PSI-reduced electron acceptors. It further establishes a cryptic role of nitrate/nitrite reductases as electron sinks to balance condition...Continue Reading

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Citations

Jul 16, 2015·The Plant Journal : for Cell and Molecular Biology·Eleonora SenderskyRakefet Schwarz
Apr 16, 2016·Applied Microbiology and Biotechnology·Kuo SongXuefeng Lu
Sep 7, 2016·Journal of Structural Biology·Frédéric DeschoenmaekerRuddy Wattiez
Mar 27, 2018·The Plant Journal : for Cell and Molecular Biology·Mali LeviRakefet Schwarz
Jun 1, 2018·Molecular & Cellular Proteomics : MCP·Philipp SpätKarl Forchhammer
Oct 16, 2018·Environmental Microbiology·Karl Forchhammer, Rakefet Schwarz
Oct 17, 2019·FEMS Microbiology Reviews·Karl Forchhammer, Khaled A Selim
Dec 2, 2020·Life·Raphaël RachediAmel Latifi
Dec 8, 2020·Frontiers in Microbiology·Hui JinZhong Hua Cai
Dec 12, 2018·Trends in Microbiology·David G WelkieSusan S Golden
May 16, 2021·Applied and Environmental Microbiology·Dana E MichelsAlexis Pasulka

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Methods Mentioned

BETA
light scattering

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