PMID: 6982070Aug 31, 1982Paper

Nitrogenase reactivity: cyanide as substrate and inhibitor

Biochemistry
J LiJ L Corbin

Abstract

We have examined the reduction of cyanide by using the purified component proteins of nitrogenase (Av1 and Av2). The previously reported self-inhibition phenomenon was found to be an artifact. One of the two species present in cyanide solutions, CN-, was shown to be a potent reversible inhibitor (Ki = 27 microM) of total electron flow, apparently uncoupling MgATP hydrolysis and electron transfer. There appears to be no differential effect of CN- on the specific activities of Av1 and Av2 nor is there any apparent irreversible physical damage to Av2. CN- inhibition is completely reversed by low levels of CO, implying a common binding site. Azide partially relieves the inhibitory effect, but other substrates and inhibitors (N2, C2H2, N2O, H2) have no effect. The other species present in cyanide solutions, HCN, was shown to be the substrate (Km = 4.5 mM at Av2/Av1 = 8), and extrapolation of the data indicates that at high enough HCN concentration H2 evolution can be eliminated. The products are methane plus ammonia (six electrons), and methylamine (four electrons). There is an excess (relative to methane) of ammonia formed, which, according to electron balance studies, may arise from a two-electron intermediate. Both nitrous oxide ...Continue Reading

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Citations

Jun 1, 1990·World Journal of Microbiology & Biotechnology·J L LegrasP Galzy
Jul 1, 2008·Applied Microbiology and Biotechnology·Lacy J BasileMichael J Benedik
May 12, 2005·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Zofia MaskosBrian J Hales
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Mar 19, 2020·Biomolecular Concepts·Kelath Murali ManojKannan Pakshirajan
Oct 17, 2013·Chemical Communications : Chem Comm·Ian Dance
Mar 8, 2017·Applied Microbiology and Biotechnology·Jason M ParkMichael J Benedik
Sep 12, 2020·Angewandte Chemie·Michael RohdeOliver Einsle
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Apr 3, 2020·Chemical Reviews·Casey Van StappenSerena DeBeer
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Oct 14, 2021·Chembiochem : a European Journal of Chemical Biology·Niels N Oehlmann, Johannes G Rebelein

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