PMID: 9436630Jan 22, 1998Paper

Nitrosylation of blood hemoglobin and renal nonheme proteins in autoimmune MRL-lpr/lpr mice

Free Radical Biology & Medicine
J B WeinbergW Chamulitrat

Abstract

MRL-lpr/lpr mice spontaneously develop manifestations of autoimmunity including arthritis, vasculitis, and glomerulonephritis. The paramagnetic molecule nitric oxide has been implicated as an effector molecule in initiation and propagation of these inflammatory conditions. In this study, we utilized electron paramagnetic resonance spectroscopy to directly detect nitrosylated protein complexes as products of nitric oxide in whole blood and in kidneys of MRL-lpr/lpr mice. Electron paramagnetic resonance spectra of blood samples from MRL-lpr/lpr mice showed nitrosyl hemoglobin species. Amounts of blood nitrosyl hemoglobin in MRL-lpr/lpr mice were significantly increased as compared to age-matched control mice. Electron paramagnetic resonance spectra of MRL-lpr/lpr kidney tissue exhibited a signal characteristic of a dinitrosyl-iron-dithiolate complex at g approximately 2.04. Formation of nitrosylated nonheme protein in diseased kidneys is associated with development of glomerulonephritis in the autoimmune mice. The presence of nitrosylated nonheme protein indicates the formation of nitric oxide within the kidneys of the diseased mice signifying in situ renal nitric oxide formation.

References

Nov 1, 1978·The Journal of Experimental Medicine·B S AndrewsF J Dixon
Apr 1, 1991·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·R D CurranT R Billiar
Oct 1, 1991·The Journal of Experimental Medicine·N S KwonC F Nathan
May 1, 1991·The American Journal of Physiology·J StadlerR L Simmons
Jan 1, 1991·Annual Review of Immunology·P L Cohen, R A Eisenberg
Jan 1, 1990·Free Radical Research Communications·U WestenbergerO Trentz
Nov 1, 1982·The Journal of Cell Biology·D L Granger, A L Lehninger
Sep 17, 1984·Biochemical and Biophysical Research Communications·J B HibbsZ Vavrin
Feb 1, 1980·The Journal of Clinical Investigation·D L GrangerJ B Hibbs
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·M A SchwarzJ R Lancaster
Nov 1, 1995·Proceedings of the Society for Experimental Biology and Medicine·R M Clancy, S B Abramson
Oct 30, 1995·FEBS Letters·Y M KimJ R Lancaster
Jan 10, 1995·Archives of Biochemistry and Biophysics·W ChamulitratL Molina y Vedia
Oct 29, 1993·Biochemical and Biophysical Research Communications·M C KennedyD H Petering
May 1, 1993·Biochemical Society Transactions·J S Beckman, J P Crow

❮ Previous
Next ❯

Citations

Dec 11, 1999·Proceedings of the Association of American Physicians·J C OatesG S Gilkeson
Jan 14, 2003·Proceedings of the National Academy of Sciences of the United States of America·Benjamin P LuchsingerDavid J Singel
Dec 24, 2011·Arthritis Research & Therapy·Tamara K Nowling, Gary S Gilkeson
Oct 28, 1998·Environmental Health Perspectives·J B Weinberg
Jun 15, 2007·Free Radical Biology & Medicine·Paolo BergamoMauro Rossi
Jun 4, 2002·Nature Medicine·Timothy J McMahonJonathan S Stamler
Jun 7, 2011·Dalton Transactions : an International Journal of Inorganic Chemistry·Hanna LewandowskaMarcin Kruszewski
Mar 29, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Kazuyoshi KirimaToshiaki Tamaki
Jul 18, 2000·American Journal of Physiology. Gastrointestinal and Liver Physiology·D M McCaffertyP Kubes

❮ Previous
Next ❯

Related Concepts

Related Feeds

Autoimmune Diseases

Autoimmune diseases occur as a result of an attack by the immune system on the body’s own tissues resulting in damage and dysfunction. There are different types of autoimmune diseases, in which there is a complex and unknown interaction between genetics and the environment. Discover the latest research on autoimmune diseases here.